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Literature summary extracted from

  • Katsu, K.; Suzuki, R.; Tsuchiya, W.; Inagaki, N.; Yamazaki, T.; Hisano, T.; Yasui, Y.; Komori, T.; Koshio, M.; Kubota, S.; Walker, A.R.; Furukawa, K.; Matsui, K.
    A new buckwheat dihydroflavonol 4-reductase (DFR), with a unique substrate binding structure, has altered substrate specificity (2017), BMC Plant Biol., 17, 239 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 expression in Escherichia coli Fagopyrum esculentum subsp. esculentum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.219 molecular docking of substrates. A phenol ring of dihydrokaempferol, a catechol ring of dihydroquercetin, and a pyrogallol ring of dihydromyricetin respectively have two, three, and four hydrogen bonds formed with side chains of residue Asn132 Fagopyrum esculentum subsp. esculentum
1.1.1.219 molecular docking of substrates. The number of hydrogen bonds is one, two, and two for the corresponding phenol, catechol, and pyrogallol rings, respectively Fagopyrum esculentum subsp. esculentum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Fagopyrum esculentum subsp. esculentum A0A2Z5XER5 isoform DFR1a
-
1.1.1.219 Fagopyrum esculentum subsp. esculentum A0A2Z5XEV7 isoform DFR2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 bud high expression level Fagopyrum esculentum subsp. esculentum
-
1.1.1.219 additional information expression in all organs investigated Fagopyrum esculentum subsp. esculentum
-
1.1.1.219 root high expression level Fagopyrum esculentum subsp. esculentum
-
1.1.1.219 root preferential expression in root and seed Fagopyrum esculentum subsp. esculentum
-
1.1.1.219 seed preferential expression in root and seed Fagopyrum esculentum subsp. esculentum
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1.1.1.219 stem high expression level in lower stem Fagopyrum esculentum subsp. esculentum
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-dihydrokaempferol + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (+)-dihydromyricetin + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (+)-dihydroquercetin + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 additional information isoform DFR1a catalyses dihydromyricetin and dihydrokaempferol with almost the same efficiency, but catalyses dihydroquercetin with a lower efficiency Fagopyrum esculentum subsp. esculentum ?
-
-
1.1.1.219 additional information isoform FeDFR2 catalyses dihydroquercetin about 2 times as efficiently as dihydromyricetin and had least activity for dihydrokaempferol Fagopyrum esculentum subsp. esculentum ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR1a
-
 Fagopyrum esculentum subsp. esculentum
1.1.1.219 DFR2
-
 Fagopyrum esculentum subsp. esculentum