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Literature summary extracted from

  • Nakahara, K.; Kitamura, Y.; Yamagishi, Y.; Shoun, H.; Yasui, T.
    Levoglucosan dehydrogenase involved in the assimilation of levoglucosan in Arthrobacter sp. I-552 (1994), Biosci. Biotechnol. Biochem., 58, 2193-2196 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.425 4-chloromercuribenzoate 1 mM, complete loss of activity Arthrobacter sp. I-552
1.1.1.425 Cd2+ 1 mM, about 30% residual activity Arthrobacter sp. I-552
1.1.1.425 Cu2+ 1 mM, about 30% residual activity Arthrobacter sp. I-552
1.1.1.425 additional information not inhibitory at 1 mM: Mg2+, Ca2+, K+, Na+, iodoacetic acid, EDTA, o-phenanthroline, ferricyanide Arthrobacter sp. I-552

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.425 0.47
-
 NAD+ pH 9, 40°C Arthrobacter sp. I-552
1.1.1.425 14
-
 levoglucosan pH 9, 40°C Arthrobacter sp. I-552

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.425 290000
-
 gel filtration Arthrobacter sp. I-552

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.425 levoglucosan + NAD+ Arthrobacter sp. I-552
-
 3-dehydrolevoglucosan + NADH + H+
-
 ?
1.1.1.425 additional information Arthrobacter sp. I-552 the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor substrates: ribose and glucose ?
-
-
1.1.1.425 sorbose + NAD+ Arthrobacter sp. I-552
-
 ? + NADH + H+
-
 ?
1.1.1.425 xylose + NAD+ Arthrobacter sp. I-552
-
 ? + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.425 Arthrobacter sp. I-552
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.425
-
 Arthrobacter sp. I-552

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.425 levoglucosan + NAD+
-
 Arthrobacter sp. I-552 3-dehydrolevoglucosan + NADH + H+
-
 ?
1.1.1.425 additional information the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor substrates: ribose and glucose Arthrobacter sp. I-552 ?
-
-
1.1.1.425 additional information the reaction follows a sequential mechanism. Catalytic activity is specific for levoglucosan and NAD+. Poor or no substrates: ribose and glucose, mannose, galactose, fructose, fucose, rhamnose, galactosan, sedoheptulosan Arthrobacter sp. I-552 ?
-
-
1.1.1.425 sorbose + NAD+
-
 Arthrobacter sp. I-552 ? + NADH + H+
-
 ?
1.1.1.425 xylose + NAD+
-
 Arthrobacter sp. I-552 ? + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.425 oligomer x * 28000 plus x * 21000, SDS-PAGE Arthrobacter sp. I-552

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.425 40
-
 assay at Arthrobacter sp. I-552
1.1.1.425 50
-
 optimum temperature Arthrobacter sp. I-552

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.425 333
-
 levoglucosan pH 9, 40°C Arthrobacter sp. I-552

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.425 9
-
-
 Arthrobacter sp. I-552

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.425 NAD+
-
 Arthrobacter sp. I-552