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Literature summary extracted from

  • Kataoka, N.; Vangnai, A.S.; Pongtharangkul, T.; Yakushi, T.; Matsushita, K.
    Production of 1,3-diols in Escherichia coli (2017), Biores. Technol., 245, 1538-1541 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.8.3.1 synthesis a platform pathway for the production of widely used industrial chemicals 1,3-diols, e.g. 1,3-pentanediol, is engineered in Escherichia coli. The pathway is designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, bld (butyraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and the endogenous alcohol dehydrogenase(s) of Escherichia coli. The recombinant Escherichia coli strains produce 1,3-pentanediol, 4-methyl-1,3-pentanediol, and 1,2,4-butanetriol, together with 1,3-butanediol, from mixtures of glucose and propionate, isobutyrate, and glycolate, respectively, in shake flask cultures Megasphaera elsdenii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.1 gene pct, recombinant coexpression with genes bktB, phaB, and bld under the control of the IPTG-inducible PA1lacO-1 promoter in Escherichia coli strain BW25113 F' Megasphaera elsdenii

Protein Variants

EC Number Protein Variants Comment Organism
2.8.3.1 additional information a platform pathway for the production of widely used industrial chemicals, 1,3-diols, is engineered in Escherichia coli strain BW25113 F'. The pathway is designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, bld (butyraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and the endogenous alcohol dehydrogenase(s) of Escherichia coli. The recombinant Escherichia coli strains produce 1,3-pentanediol, 4-methyl-1,3-pentanediol, and 1,2,4-butanetriol, together with 1,3-butanediol, from mixtures of glucose and propionate, isobutyrate, and glycolate, respectively, in shake flask cultures. Production of 1,3-pentanediol Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.1 acetyl-CoA + propanoate Megasphaera elsdenii
-
 acetate + propanoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.1 Megasphaera elsdenii A0A140JTF0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.1 acetyl-CoA + glycolate
-
 Megasphaera elsdenii acetate + glycolyl-CoA
-
 ?
2.8.3.1 acetyl-CoA + isobutyrate
-
 Megasphaera elsdenii acetate + isobutanoyl-CoA
-
 ?
2.8.3.1 acetyl-CoA + lactate
-
 Megasphaera elsdenii acetate + lactoyl-CoA
-
 ?
2.8.3.1 acetyl-CoA + propanoate
-
 Megasphaera elsdenii acetate + propanoyl-CoA
-
 ?
2.8.3.1 additional information although propionate is a natural substrate for Pct, Pct is active toward several short-chain organic acids such as isobutyrate, glycolate, and lactate Megasphaera elsdenii ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.8.3.1 PCT
-
 Megasphaera elsdenii

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.3.1 acetyl-CoA
-
 Megasphaera elsdenii