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Literature summary extracted from

  • Song, P.; Wang, M.; Zheng, Q.; Wang, P.; Zhu, G.
    Isocitrate dehydrogenase 1 from Acinetobacter baummanii (AbIDH1) enzymatic characterization and its regulation by phosphorylation (2021), Biochimie, 181, 77-85 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressipon in Escherichia coli Acinetobacter baumannii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 S113A mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
1.1.1.42 S113A the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
1.1.1.42 S113D the mutant lacks almost all enzymatic activity Acinetobacter baumannii
1.1.1.42 S113D the phosphorylation-mimicking mutant lacks almost all enzymatic activity Acinetobacter baumannii
1.1.1.42 S113E the mutant lacks almost all enzymatic activity Acinetobacter baumannii
1.1.1.42 S113E the phosphorylation-mimicking mutant lacks almost all enzymatic activity Acinetobacter baumannii
1.1.1.42 S113G mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
1.1.1.42 S113G the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
1.1.1.42 S113T mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
1.1.1.42 S113T the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
1.1.1.42 S113Y mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
1.1.1.42 S113Y the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii

General Stability

EC Number General Stability Organism
1.1.1.42 the activity of the enzyme treated with Escherichia coli isocitrate dehydrogenase kinase/phosphatase immediately decreases by 80% within 9 min Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0181
-
 NADP+ in the presence of Mg2+, pH and temperature not specified in the publication Acinetobacter baumannii
1.1.1.42 0.0181
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 0.0223
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 0.0261
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 0.0289
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 0.0466
-
 NADP+ in the presence of Mn2+, pH and temperature not specified in the publication Acinetobacter baumannii
1.1.1.42 0.0466
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 0.0505
-
 isocitrate in the presence of Mn2+, pH and temperature not specified in the publication Acinetobacter baumannii
1.1.1.42 0.0505
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 0.0654
-
 isocitrate in the presence of Mg2+, pH and temperature not specified in the publication Acinetobacter baumannii
1.1.1.42 0.0654
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 0.0879
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 0.1043
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 0.1169
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 0.1221
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 0.1414
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 0.2447
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 0.3276
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 0.8546
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
1.1.1.42 2.525
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ activates Acinetobacter baumannii
1.1.1.42 Mn2+ activates Acinetobacter baumannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 83500
-
 gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Acinetobacter baumannii
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+ Acinetobacter baumannii overall reaction 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] Escherichia coli
-
 ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Acinetobacter baumannii
-
-
-
1.1.1.42 Acinetobacter baumannii V5VCI0
-
-
2.7.11.5 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.42 phosphoprotein the enzyme is phosphorylated at Ser113 by Escherichia coli isocitrate dehydrogenase kinase/phosphatase Acinetobacter baumannii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+ overall reaction Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 additional information the enzyme is a substrate for Escherichia coli isocitrate dehydrogenase kinase/phosphatase Acinetobacter baumannii ?
-
-
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)]
-
 Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 ?
2.7.11.5 ATP + [isocitrate dehydrogenase (NADP+)] isocitrate dehydrogenase (NADP+) from Acinetobacter baumannii (AbIDH1) is a substrate for the enzyme (IDHKP) from Escherichia coli. The conserved Ser113 of AbIDH1 is phosphorylated Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 homodimer 2 * 45000, SDS-PAGE Acinetobacter baumannii
1.1.1.42 homodimer 2 * 83500, SDS-PAGE Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 AbIDH1
-
 Acinetobacter baumannii
1.1.1.42 IDH1
-
 Acinetobacter baumannii
1.1.1.42 isocitrate dehydrogenase 1
-
 Acinetobacter baumannii
2.7.11.5 IDHKP
-
 Escherichia coli
2.7.11.5 isocitrate dehydrogenase kinase/phosphatase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 2
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 2.1
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 2.9
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 3 6 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 3.2
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 3.4
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
1.1.1.42 4
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
1.1.1.42 28.5
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 29.2
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 29.8
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 30.3
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 31.3
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 33.4
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 37
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 50.4
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 60.1
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ dependent on Acinetobacter baumannii
1.1.1.42 NADP+ NADP+-dependent enzyme Acinetobacter baumannii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 1
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 1.1
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 1.3
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 2
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
1.1.1.42 4
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
1.1.1.42 6
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 13
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 16
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
1.1.1.42 33
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
1.1.1.42 250
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1.1.42 300
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.1.1.42 320
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
1.1.1.42 500
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 1000
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 1300
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1.1.1.42 1700
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii