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Literature summary extracted from

  • Li, C.C.; Yang, M.J.; Liu, L.; Li, T.; Peng, C.T.; He, L.H.; Song, Y.J.; Zhu, Y.B.; Shen, Y.L.; Yang, J.; Zhao, N.L.; Zhao, C.; Zhou, Q.X.; Li, H.; Kang, M.; Tong, A.P.; Tang, H.; Bao, R.
    Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase (2018), Biochem. J., 475, 1107-1119 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.4 hanging drop vapor diffusion method, using 0.4 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate (pH 5.6) and 0.9 M lithium sulfate monohydrate Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.4 D182/R184A the mutations decrease the enzyme activities to about 65% Pseudomonas aeruginosa
2.7.2.4 G152L the mutant shows about 35% activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.2.4 H399A the mutant shows wild type activity Pseudomonas aeruginosa
2.7.2.4 N371A/I372A the mutations do not affect the ATP binding with threonine Pseudomonas aeruginosa
2.7.2.4 R150A the mutant shows about 30% activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.2.4 S12C/S231C the mutant shows wild type activity Pseudomonas aeruginosa
2.7.2.4 S378A/E202A the mutant shows slightly increased activity (about 108%) compared to the wild type enzyme Pseudomonas aeruginosa
2.7.2.4 S378A/E202A the mutations do not affect the ATP binding with threonine Pseudomonas aeruginosa
2.7.2.4 V357/M351A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 lysine
-
 Pseudomonas aeruginosa
2.7.2.4 threonine
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 Mg2+ 10 mM used in assay conditions Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Pseudomonas aeruginosa
-
 ADP + 4-phospho-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Pseudomonas aeruginosa O69077
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 Ni-NTA resin column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Pseudomonas aeruginosa ADP + 4-phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 heterotetramer x-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 aspartokinase
-
 Pseudomonas aeruginosa
2.7.2.4 lysC
-
 Pseudomonas aeruginosa