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Literature summary extracted from

  • Blaise, M.; Van Wyk, N.; Baneres-Roquet, F.; Guerardel, Y.; Kremer, L.
    Binding of NADP+ triggers an open-to-closed transition in a mycobacterial FabG beta-ketoacyl-ACP reductase (2017), Biochem. J., 474, 907-921 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.36 expressed in Escherichia coli BL21 Rosetta2 (DE3) pLysS cells and a fabG -temperature-sensitive mutant Escherichia coli CL104 strain Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.36 apo and NADP+-bound enzyme forms, sitting drop vapor diffusion method, using 100 mM Na-HEPES (pH 7.5), 30% (v/v) PEG 400 and 200 mM magnesium chloride hexahydrate for the apo enzyme and 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 for the NADP+-bound enzyme Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.36 100000
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Mycolicibacterium smegmatis
-
 (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+ Mycolicibacterium smegmatis mc2155
-
 (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.36 Mycolicibacterium smegmatis A0R723
-
-
1.1.1.36 Mycolicibacterium smegmatis mc2155 A0R723
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.36 nickel Sepharose bead chromatography Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.36 0.025
-
 at pH 7.0 and 25°C Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Mycolicibacterium smegmatis (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 acetoacetyl-CoA + NADPH + H+
-
 Mycolicibacterium smegmatis mc2155 (R)-3-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.36 additional information no activity is detected when NADPH is replaced by NADH Mycolicibacterium smegmatis ?
-
-
1.1.1.36 additional information no activity is detected when NADPH is replaced by NADH Mycolicibacterium smegmatis mc2155 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.36 homotetramer 4 * 25000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.36 beta-ketoacyl reductase
-
 Mycolicibacterium smegmatis
1.1.1.36 ketoacyl reductase
-
 Mycolicibacterium smegmatis
1.1.1.36 MSMEG_6753
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.36 NADPH
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
1.1.1.36 physiological function the enzyme is dispensable for mycobacterial growth Mycolicibacterium smegmatis