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Literature summary extracted from

  • Zhou, W.; Huang, R.; Zhu, Z.; Zhang, Y.P.J.
    Coevolution of both Thermostability and activity of polyphosphate glucokinase from Thermobifida fusca YX (2018), Appl. Environ. Microbiol., 84, e01224-18 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.63 synthesis great potentials of directed evolution in obtaining high-performance BioBricks suitable for the in vitro synthetic biology platform Thermobifida fusca

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.63 expression in Escherichia coli BL21(DE3) Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.63 A231T 2.8fold increase in t1/2 at 55°C Thermobifida fusca
2.7.1.63 F30L/R34P/T72A/V88M/A231T 7200fold increase in t1/2 at 55°C Thermobifida fusca
2.7.1.63 F30L/T72A/A231T 185fold increase in t1/2 at 55°C Thermobifida fusca
2.7.1.63 F30LT72A/V88M/A231T 1570fold increase in t1/2 at 55°C Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose Thermobifida fusca
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Thermobifida fusca Q47NX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63
-
 Thermobifida fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.63 285.3
-
 pH 7.5, 50°C Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose
-
 Thermobifida fusca (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.63 PPGK
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.63 50
-
 wild-type enzyme and mutant enzyme A231T Thermobifida fusca
2.7.1.63 55
-
 mutant enzyme F30L/T72A/A231T Thermobifida fusca
2.7.1.63 60
-
 mutant enzyme F30L/T72A/V88M/A231T Thermobifida fusca
2.7.1.63 65
-
 mutant enzyme F30L/R34P/T72A/V88M/A231T Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.63 55
-
 t1/2: 1.4 min (wild-type enzyme), t1/2: 3.2 min (mutant enzyme A231T), t1/2: 240 min (mutant enzyme F30L/T72A/A231T), t1/2: 2200 min (mutant enzyme F30L/T72A/V88M/A231T), t1/2: 10080 min (mutant enzyme F30L/R34P/T72A/V88M/A231T) Thermobifida fusca
2.7.1.63 55.9
-
 Tm-value, wild-type enzyme Thermobifida fusca
2.7.1.63 59.1
-
 Tm-value, mutant enzyme A231T Thermobifida fusca
2.7.1.63 65.2
-
 Tm-value, mutant enzyme F30L/T72A/A231T Thermobifida fusca
2.7.1.63 70.6
-
 Tm-value, mutant enzyme F30L/T72A/V88M/A231T Thermobifida fusca
2.7.1.63 75.7
-
 Tm-value, mutant enzyme F30L/R34P/T72A/V88M/A231T Thermobifida fusca

General Information

EC Number General Information Comment Organism
2.7.1.63 evolution coevolution of both the thermostability and catalytic activity of the enzyme by directed evolution Thermobifida fusca