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Literature summary extracted from

  • Kamenskikh, K.; Vekshin, N.
    Reactions of NADH oxidation by tetrazolium and ubiquinone catalyzed by yeast alcohol dehydrogenase (2018), Appl. Biochem. Microbiol., 54, 316-319 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 ethanol presence of ethanol or isopropanol and alkalization of the medium sharply activates the NADH:p-NTF-reductase reaction, activity with ubiquinone is also activated Saccharomyces cerevisiae
1.1.1.1 Isopropanol presence of ethanol or isopropanol and alkalization of the medium sharply activates the NADH:p-NTF-reductase reaction, activity with ubiquinone is also activated Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 additional information
-
 additional information reaction kinetics at different pH values, overview Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn2+ required Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharomyces cerevisiae P00330
-
-
1.1.1.1 Saccharomyces cerevisiae ATCC 204508 P00330
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 commercial preparation
-
 Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 4-nitrotetrazolium violet + NADH + H+ p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF Saccharomyces cerevisiae a formazan + NAD+
-
 ?
1.1.1.1 4-nitrotetrazolium violet + NADH + H+ p-NTF, the tetrazolium contacts with NADH on the enzyme surface without intermediate carriers (the zinc in the active ADH site is not a electron carrier) and accepts electrons. Alcohols are not able to reduce p-NTF Saccharomyces cerevisiae ATCC 204508 a formazan + NAD+
-
 ?
1.1.1.1 additional information a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules Saccharomyces cerevisiae ?
-
-
1.1.1.1 additional information a quasi-vibrational process is detected with the use of HEPES buffer in the presence of alcohol: NADH is rapidly oxidized to NAD+ by ubiquinone and NAD+ is then slowly reduced to NADH by the alcohol. The NADH:ubiquinone-and alcohol:NAD+-reductase reactions are partially separated in time caused by considerable differences in the values of binding constants of NADH and NAD+ molecules Saccharomyces cerevisiae ATCC 204508 ?
-
-
1.1.1.1 ubiquinone + NADH + H+
-
 Saccharomyces cerevisiae ubiquinol + NAD+
-
 ?
1.1.1.1 ubiquinone + NADH + H+
-
 Saccharomyces cerevisiae ATCC 204508 ubiquinol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Saccharomyces cerevisiae
1.1.1.1 ADH1
-
 Saccharomyces cerevisiae
1.1.1.1 NADH:p-NTF-reductase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 20
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 8 9
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 5.5 9
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NADH
-
 Saccharomyces cerevisiae