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Literature summary extracted from

  • Gatreddi, S.; Are, S.; Qureshi, I.A.
    Ribokinase from Leishmania donovani purification, characterization and X-ray crystallographic analysis (2018), Acta Crystallogr. Sect. F, 74, 99-104 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.15 expression in Escherichia coli BL21 (DE3) cells Leishmania donovani

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.15 crystals obtained by the hanging-drop vapour-diffusion method at 23°C diffract to 1.95 A resolution and belong to the hexagonal space group P61, with unit-cell parameters a = b = 100.25, c = 126.77 A Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.15 0.116
-
 ATP pH 8.5, 25°C Leishmania donovani
2.7.1.15 0.296
-
 D-ribose pH 8.5, 25°C Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.15 38000
-
 SDS-PAGE Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.15 ATP + D-ribose Leishmania donovani the enzyme catalyzes the first step of ribose metabolism ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose Leishmania donovani BPK282A1 the enzyme catalyzes the first step of ribose metabolism ADP + D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.15 Leishmania donovani E9BIX7
-
-
2.7.1.15 Leishmania donovani BPK282A1 E9BIX7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.15 Ni2+-affinity and gel-filtration chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.15 ATP + D-ribose
-
 Leishmania donovani ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose the enzyme catalyzes the first step of ribose metabolism Leishmania donovani ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose
-
 Leishmania donovani BPK282A1 ADP + D-ribose 5-phosphate
-
 ?
2.7.1.15 ATP + D-ribose the enzyme catalyzes the first step of ribose metabolism Leishmania donovani BPK282A1 ADP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.15 ? x * 38000, SDS-PAGE Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.15 25
-
 assay at Leishmania donovani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.15 44
-
 Tm-value Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.15 2.15
-
 ATP pH 8.5, 25°C Leishmania donovani
2.7.1.15 2.28
-
 D-ribose pH 8.5, 25°C Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.15 8.5
-
 assay at Leishmania donovani

General Information

EC Number General Information Comment Organism
2.7.1.15 physiological function the enzyme catalyzes the first step of ribose metabolism Leishmania donovani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.15 7.7
-
 D-ribose pH 8.5, 25°C Leishmania donovani
2.7.1.15 18.5
-
 ATP pH 8.5, 25°C Leishmania donovani