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Literature summary extracted from

  • Saito, T.; Takahashi, D.; Sakane, F.
    Expression, purification, and characterization of human diacylglycerol kinase zeta (2019), ACS Omega, 4, 5540-5546 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 phosphatidylserine
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 using a baculovirus-Sf9 insect cell expression system Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.05
-
 ATP pH and temperature not specified in the publication Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 120000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Homo sapiens
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens Q13574
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 Ni-NTA agarose column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dilauroyl-sn-glycerol high activity Homo sapiens ADP + 1,2-dilauroyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dioleoyl-sn-glycerol high activity Homo sapiens ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dipalmitoyl-sn-glycerol highest activity Homo sapiens ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-distearoyl-sn-glycerol high activity Homo sapiens ADP + 1,2-distearoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-palmitoyl-2-oleolyl-sn-glycerol high activity Homo sapiens ADP + 1-palmitoyl-2-oleolyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol high activity Homo sapiens ADP + 1-stearoyl-2-docosahexaenoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1-stearoyl-2-eicosatetraenoyl-sn-glycerol high activity Homo sapiens ADP + 1-stearoyl-2-eicosatetraenoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 monomer 1 * 106000, His6-tagged enzyme, calculated from amino acid sequence Homo sapiens
2.7.1.107 monomer 1 * 125000, His6-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGKzeta
-
 Homo sapiens
2.7.1.107 diacylglycerol kinase zeta
-
 Homo sapiens