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Literature summary extracted from

  • Wang, C.C.; Chen, B.H.; Lu, L.Y.; Hung, K.S.; Yang, Y.S.
    Preparation of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides (2018), ACS Omega, 3, 11633-11642 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.8.2.20 synthesis usage of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides Homo sapiens
2.8.2.20 synthesis usage of tyrosylprotein sulfotransferases for in vitro one-pot enzymatic synthesis of sulfated proteins/peptides Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.20 gene Tpst, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview Drosophila melanogaster
2.8.2.20 gene TPST1, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview Homo sapiens
2.8.2.20 gene TPST2, recombinant expression of His-tagged enzyme fused to GST or NusA in Escherichia coli strain BL21(DE3), method optimization, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 Golgi membrane
-
 Homo sapiens 139
-
2.8.2.20 Golgi membrane
-
 Drosophila melanogaster 139
-
2.8.2.20 membrane a type II membrane enzyme Homo sapiens 16020
-
2.8.2.20 membrane a type II membrane enzyme Drosophila melanogaster 16020
-
2.8.2.20 trans-Golgi network
-
 Homo sapiens 5802
-
2.8.2.20 trans-Golgi network
-
 Drosophila melanogaster 5802
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + protein tyrosine Homo sapiens
-
 adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + protein tyrosine Drosophila melanogaster
-
 adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Drosophila melanogaster Q9VYB7
-
-
2.8.2.20 Homo sapiens O60507
-
-
2.8.2.20 Homo sapiens O60704
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.20 preparation of TPST from Golgi-enriched membrane fractions, recombinant His-tagged GST- or NusA-fused enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, low solubility or contamination with tag material Homo sapiens
2.8.2.20 preparation of TPST from Golgi-enriched membrane fractions, recombinant His-tagged GST- or NusA-fused enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, low solubility or contamination with tag material Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag Homo sapiens ?
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 recombinantly expressed substrate PSGL-1, glycoprotein found on the plasma membrane of neutrophils or monocytes. TPST activity on GST-tagged PSGL-1 is not affected by the GST fusion tag Drosophila melanogaster ?
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + protein tyrosine
-
 Homo sapiens adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?
2.8.2.20 3'-phosphoadenylyl sulfate + protein tyrosine
-
 Drosophila melanogaster adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.20 DmTPST
-
 Homo sapiens
2.8.2.20 DmTPST
-
 Drosophila melanogaster
2.8.2.20 TPST
-
 Homo sapiens
2.8.2.20 TPST
-
 Drosophila melanogaster
2.8.2.20 TPST-1
-
 Homo sapiens
2.8.2.20 TPST-2
-
 Homo sapiens
2.8.2.20 TPST1
-
 Homo sapiens
2.8.2.20 TPST2
-
 Homo sapiens
2.8.2.20 tyrosylprotein sulfotransferase
-
 Homo sapiens
2.8.2.20 tyrosylprotein sulfotransferase
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.8.2.20 physiological function protein tyrosine sulfation (PTS), catalyzed by membrane-anchored tyrosylprotein sulfotransferase (TPST), is one of the most common posttranslational modifications of secretory and transmembrane proteins. PTS, a key modulation of extracellular protein-protein interactions, accounts for various important biological activities, namely, virus entry, inflammation, coagulation, and sterility. PTS occurs in the trans-Golgi network, and the sulfated proteins and peptides are typically transmembrane or secretory proteins Homo sapiens
2.8.2.20 physiological function protein tyrosine sulfation (PTS), catalyzed by membrane-anchored tyrosylprotein sulfotransferase (TPST), is one of the most common posttranslational modifications of secretory and transmembrane proteins. PTS, a key modulation of extracellular protein-protein interactions, accounts for various important biological activities, namely, virus entry, inflammation, coagulation, and sterility. PTS occurs in the trans-Golgi network, and the sulfated proteins and peptides are typically transmembrane or secretory proteins Drosophila melanogaster