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Literature summary extracted from

  • Nocek, B.; Khusnutdinova, A.; Ruszkowski, M.; Flick, R.; Burda, M.; Batyrova, K.; Brown, G.; Mucha, A.; Joachimiak, A.; Berlicki, L.; Yakunin, A.
    Structural insights into substrate selectivity and activity of bacterial polyphosphate kinases (2018), ACS Catal., 8, 10746-10760 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1
-
 Sinorhizobium meliloti
2.7.4.1
-
 Cytophaga hutchinsonii
2.7.4.1
-
 Deinococcus radiodurans
2.7.4.1
-
 Paenarthrobacter aurescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.1 sitting-drop vapor-diffusion method at 18°C Sinorhizobium meliloti
2.7.4.1 sitting-drop vapor-diffusion method at 18°C Deinococcus radiodurans
2.7.4.1 sitting-drop vapor-diffusion method at 18°C Paenarthrobacter aurescens
2.7.4.1 sitting-drop vapor-diffusion method at 18°C, crystal structures of CHU0107 in complex with AMP, ADP, or guanosine 5'-tetraphosphate. The structures of CHU0107-inhibitor complexes reveales at least two binding pockets for aryl phosphonate inhibitors Cytophaga hutchinsonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 guanosine 5'-tetraphosphate
-
 Cytophaga hutchinsonii
2.7.4.1 guanosine 5'-tetraphosphate
-
 Deinococcus radiodurans
2.7.4.1 guanosine 5'-tetraphosphate
-
 Paenarthrobacter aurescens
2.7.4.1 guanosine 5'-tetraphosphate
-
 Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.011
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
2.7.4.1 0.013
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans
2.7.4.1 0.11
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.17
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.2
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.27
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.29
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
2.7.4.1 0.35
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.53
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.62
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.71
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.83
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 1.03
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 1.69
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 2.71
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 2.74
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 2.88
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 3.15
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 8.64
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 17.7
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 39.2
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Cytophaga hutchinsonii A0A6N4SMB5
-
-
2.7.4.1 Cytophaga hutchinsonii ATCC 33406 A0A6N4SMB5
-
-
2.7.4.1 Deinococcus radiodurans Q9RY20
-
-
2.7.4.1 Deinococcus radiodurans ATCC 13939 Q9RY20
-
-
2.7.4.1 Paenarthrobacter aurescens A1R8G0
-
-
2.7.4.1 Paenarthrobacter aurescens TC1 A1R8G0
-
-
2.7.4.1 Sinorhizobium meliloti Q92SA6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1
-
 Sinorhizobium meliloti
2.7.4.1
-
 Cytophaga hutchinsonii
2.7.4.1
-
 Deinococcus radiodurans
2.7.4.1
-
 Paenarthrobacter aurescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1
-
 Deinococcus radiodurans ATP + (phosphate)n
-
 ?
2.7.4.1 ADP + (phosphate)n+1
-
 Paenarthrobacter aurescens ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 ATP + (phosphate)n
-
 r
2.7.4.1 ADP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 ATP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Cytophaga hutchinsonii ADP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Deinococcus radiodurans ADP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Paenarthrobacter aurescens ADP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 ADP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 ADP + (phosphate)n
-
 ?
2.7.4.1 AMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 ADP + (phosphate)n
-
 ?
2.7.4.1 ATP + (phosphate)n
-
 Cytophaga hutchinsonii ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Paenarthrobacter aurescens ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Cytophaga hutchinsonii ATCC 33406 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Paenarthrobacter aurescens TC1 ADP + (phosphate)n+1
-
 r
2.7.4.1 dADP + (phosphate)n+1
-
 Cytophaga hutchinsonii dATP + (phosphate)n
-
 ?
2.7.4.1 dADP + (phosphate)n+1
-
 Paenarthrobacter aurescens dATP + (phosphate)n
-
 ?
2.7.4.1 dADP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 dATP + (phosphate)n
-
 ?
2.7.4.1 dADP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 dATP + (phosphate)n
-
 ?
2.7.4.1 dAMP + (phosphate)n+1
-
 Cytophaga hutchinsonii dADP + (phosphate)n
-
 ?
2.7.4.1 dAMP + (phosphate)n+1
-
 Deinococcus radiodurans dADP + (phosphate)n
-
 ?
2.7.4.1 dAMP + (phosphate)n+1
-
 Paenarthrobacter aurescens dADP + (phosphate)n
-
 ?
2.7.4.1 dAMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 dADP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1
-
 Cytophaga hutchinsonii GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1
-
 Paenarthrobacter aurescens GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1
-
 Cytophaga hutchinsonii ATCC 33406 GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1
-
 Paenarthrobacter aurescens TC1 GTP + (phosphate)n
-
 ?
2.7.4.1 GMP + (phosphate)n+1
-
 Cytophaga hutchinsonii GDP + (phosphate)n
-
 ?
2.7.4.1 GMP + (phosphate)n+1
-
 Deinococcus radiodurans GDP + (phosphate)n
-
 ?
2.7.4.1 GMP + (phosphate)n+1
-
 Paenarthrobacter aurescens GDP + (phosphate)n
-
 ?
2.7.4.1 GMP + (phosphate)n+1
-
 Deinococcus radiodurans ATCC 13939 GDP + (phosphate)n
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 AAur2811
-
 Paenarthrobacter aurescens
2.7.4.1 CHU0107
-
 Cytophaga hutchinsonii
2.7.4.1 DR0132
-
 Deinococcus radiodurans
2.7.4.1 PPK2
-
 Sinorhizobium meliloti
2.7.4.1 PPK2
-
 Cytophaga hutchinsonii
2.7.4.1 PPK2
-
 Deinococcus radiodurans
2.7.4.1 PPK2
-
 Paenarthrobacter aurescens
2.7.4.1 SMc02148
-
 Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 0.06
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.29
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.4
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.55
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.58
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.6
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.8
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.97
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 2.12
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 2.77
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 3.32
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 3.86
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 4.12
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 5.53
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
2.7.4.1 6.91
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 9.39
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 16.83
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 21.24
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 29.63
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
2.7.4.1 94.47
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 132
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.1 0.1
-
 ATP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 0.1
-
 GDP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.1
-
 GMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.1
-
 dAMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.3
-
 GMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 0.4
-
 ATP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 0.6
-
 dAMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 1.1
-
 dADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 1.4
-
 GDP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 1.6
-
 dAMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 2
-
 ADP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 2.2
-
 GMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 5
-
 dADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 24
-
 ADP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 26
-
 AMP pH 8.0, 30°C Paenarthrobacter aurescens
2.7.4.1 27
-
 ADP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 27
-
 AMP pH 8.0, 30°C Cytophaga hutchinsonii
2.7.4.1 100
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Cytophaga hutchinsonii
2.7.4.1 350
-
 AMP pH 8.0, 30°C Deinococcus radiodurans
2.7.4.1 500
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Paenarthrobacter aurescens
2.7.4.1 10200
-
 (phosphate)n+1 pH 8.0, 30°C, cosubstrate: ADP Deinococcus radiodurans