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Literature summary extracted from
- Crystal structure of exotoxin A from Aeromonas pathogenic species (2020), Toxins, 12, 397 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.2.36 | expressed in Escherichia coli K-12 cells | Aeromonas hydrophila |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 2.4.2.36 | hanging drop vapor diffusion method, using 12% (w/v) polyethylene glycol 6000, 0.1 M MES pH 5.5, 0.1 M ammonium acetate | Aeromonas hydrophila |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.4.2.36 | E571A | catalytically inactive | Aeromonas hydrophila |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.36 | NAD+ + diphthamide-[translation elongation factor 2] | Aeromonas hydrophila | - |
nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.2.36 | Aeromonas hydrophila | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.2.36 | HisTrap column chromatography and Superdex S200 gel filtration | Aeromonas hydrophila |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.36 | NAD+ + diphthamide-[translation elongation factor 2] | - |
Aeromonas hydrophila | nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.2.36 | ? | x * 67000, SDS-PAGE | Aeromonas hydrophila |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.2.36 | diphthamide-specific mono-ADP-ribosylating toxin | - |
Aeromonas hydrophila |
| 2.4.2.36 | exotoxin A | - |
Aeromonas hydrophila |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.4.2.36 | physiological function | the enzyme is an important virulence factor from Aeromonas pathogenic species | Aeromonas hydrophila |
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Release 2023.1 (January 2023)
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