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Literature summary extracted from

  • Zhang, D.; Yamamoto, T.; Tang, D.; Kato, Y.; Horiuchi, S.; Ogawa, S.; Yoshimura, E.; Suzuki, M.
    Enhanced biosynthesis of CdS nanoparticles through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid (2019), Talanta, 195, 447-455 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.15 biotechnology enhanced biosynthesis of cadmium(II)-selenide (CdS) nanoparticles (NPs) through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli with fluorescence effect in detection of pyrogallol and gallic acid. CdS NPs act as a classical II-VI semiconductor with various interesting properties and incomparable advantages. Enzyme PCS can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs will be greatly improved. A direct detection method based on such CdS NPs is established for determination of pyrogallol and gallic acid, two organic molecules with multiple advantages and wide applications. The method provides an excellent alternative to traditional chemosynthesis and quantitative analysis, which also displays great potential for technological improvement towards green synthesis and application. Method development and evaluation, detailed overview Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 gene PCS1, recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Arabidopsis thaliana Q9S7Z3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?
2.3.2.15 additional information the PCS enzyme catalyzes transformation of GSH to give phytochelatins (PCs), it can effectively capture and enrich Cd2+ so that the generation and subsequent application process of CdS NPs is greatly improved Arabidopsis thaliana ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.2.15 ? x * 54500, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 AtPCS1
-
 Arabidopsis thaliana
2.3.2.15 PCS
-
 Arabidopsis thaliana
2.3.2.15 PCS1
-
 Arabidopsis thaliana
2.3.2.15 phytochelatin synthase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.15 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.15 8
-
 assay at Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.15 3 8 activity range Arabidopsis thaliana