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Literature summary extracted from

  • Sasidharan, S.; Saudagar, P.
    Mapping N- and C-terminals of Leishmania donovani tyrosine aminotransferase by gene truncation strategy a functional study using in vitro and in silico approaches (2020), Sci. Rep., 10, 12463 .
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.6.1.5 activity of the N-terminal domain in the presence of urea is recorded till 2.5 M urea, after which negligible activity is recorded till 8 M urea concentration. In the case of the C-terminal domain, the activity of the protein gradually decreases till 2 M urea, and no further activity is detected from 3 M urea concentration. Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 3.468
-
 L-tyrosine N-terminal domain, with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 4.581
-
 L-tyrosine C-terminal domain, with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.5 L-tyrosine + 2-oxoglutarate Leishmania donovani
-
 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Leishmania donovani A0A5B8TZA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Leishmania donovani 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + pyruvate
-
 Leishmania donovani 4-hydroxyphenylpyruvate + L-alanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.5 ? x * 43000, N-terminal domain, SDS-PAGE Leishmania donovani
2.6.1.5 ? x * 45000, C-terminal domain, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 L-tyrosine: 2 oxoglutarate aminotransferase
-
 Leishmania donovani
2.6.1.5 TAT
-
 Leishmania donovani
2.6.1.5 TATase
-
 Leishmania donovani
2.6.1.5 tyrosine aminotransferase
-
 Leishmania donovani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.5 20 70 the activity of the full-length enzyme as well as its truncated N-terminal domain remains stable from 20-70°C, and then gradually decreases until 100°C. The truncated C-terminal domain displays its highest activity at 30-40°C but the activity decreases drastically at temperatures higher than 40°C Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8
-
-
 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.5 4 9 more than 50% activity between pH 4.0 and 9.0 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.5 pyridoxal 5'-phosphate
-
 Leishmania donovani