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Literature summary extracted from

  • Liu, Y.; Hu, H.; Wang, J.; Zhou, Q.; Wu, P.; Yan, N.; Wang, H.; Wu, J.; Sun, L.
    Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis (2019), Protein Cell, 10, 365-369 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.52 A723R the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 D594A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 D601A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 D762A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G597A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G598A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G713A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 G715A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 G717A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 G757A the mutant has greatly reduced fucokinase activity compared to the wild type enzyme Bacteroides fragilis
2.7.1.52 G758A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G759A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G899A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G901A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 G902A the mutant has greatly reduced fucokinase activity compared to the wild type enzyme Bacteroides fragilis
2.7.1.52 Q761A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 R592A the mutant retains hardly any fucokinase activity Bacteroides fragilis
2.7.1.52 S714A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 S719A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 S720A the fucokinase activity of the mutant is totally abolished Bacteroides fragilis
2.7.1.52 T602A the mutant has greatly reduced fucokinase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 A723R the mutant shows about 75% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 D594A the mutant shows about 55% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 D601A the mutant shows about 40% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 D762A the mutant shows about 85% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G597A the mutant shows about 20% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G598A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G713A the mutant shows about 125% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G715A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G717A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G757A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G758A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G759A the mutant shows about 85% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G75A the mutant shows about 60% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G76A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G88A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 G899A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G901A the mutant shows about 87% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 G902A the mutant shows about 80% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 H73A the mutant shows about 40% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 I721A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 K89A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 L722A the mutant shows about 120% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 P711A the mutant shows about 120% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 R592A the mutant shows about 110% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 R80A the mutant almost completely loses its GDP-fucose pyrophosphorylase activity Bacteroides fragilis
2.7.7.30 R82A the mutant shows about 50% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 S714A the mutant shows about 130% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 S719A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 S720A the mutant shows about 75% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 S78A the mutant shows about 80% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 T602A the mutant shows about 90% GDP-fucose pyrophosphorylase activity compared to the wild type enzyme Bacteroides fragilis
2.7.7.30 T718A the mutant shows wild type GDP-fucose pyrophosphorylase activity Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.06629
-
 L-fucose pH and temperature not specified in the publication Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.30 Mg2+ required Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 338000
-
 ultracentrifugation sedimentation velocity analysis Bacteroides fragilis
2.7.1.52 440000
-
 gel filtration Bacteroides fragilis
2.7.7.30 338000
-
 ultracentrifugation sedimentation velocity analysis Bacteroides fragilis
2.7.7.30 440159
-
 gel filtration Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + L-fucose Bacteroides fragilis
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose Bacteroides fragilis 9343
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate Bacteroides fragilis
-
 diphosphate + GDP-L-fucose
-
 r
2.7.7.30 GTP + beta-L-fucose 1-phosphate Bacteroides fragilis 9343
-
 diphosphate + GDP-L-fucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Bacteroides fragilis Q58T34
-
-
2.7.1.52 Bacteroides fragilis 9343 Q58T34
-
-
2.7.7.30 Bacteroides fragilis
-
-
-
2.7.7.30 Bacteroides fragilis 9343
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + L-fucose
-
 Bacteroides fragilis ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose
-
 Bacteroides fragilis 9343 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis diphosphate + GDP-L-fucose
-
 r
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis 9343 diphosphate + GDP-L-fucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.52 homotetramer 4 * 105000, calculated from amino acid sequence Bacteroides fragilis
2.7.7.30 homotetramer 4 * 105000, calculated from amino acid sequence Bacteroides fragilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.52 FKP bifunctional enzyme with L-fucokinase/GDP-fucose diphosphorylase activities Bacteroides fragilis
2.7.1.52 L-fucokinase
-
 Bacteroides fragilis
2.7.7.30 FKP bifunctional enzyme with L-fucokinase and GDP-fucose pyrophosphorylase activities Bacteroides fragilis
2.7.7.30 GDP-fucose pyrophosphorylase
-
 Bacteroides fragilis
2.7.7.30 GFPP
-
 Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.52 0.39
-
 L-fucose pH and temperature not specified in the publication Bacteroides fragilis