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Literature summary extracted from

  • Emiola, A.; Andrews, S.; Heller, C.; George, J.
    Crosstalk between the lipopolysaccharide and phospholipid pathways during outer membrane biogenesis in Escherichia coli (2016), Proc. Natl. Acad. Sci. USA, 113, 3108-3113 .
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.1.130 palmitoyl-CoA induces a strong inhibitory effect on FabI. Due to the inhibition of FabI, LpxC would be stabilized Escherichia coli
3.5.1.108 palmitoyl-CoA induces a strong inhibitory effect on FabI. Due to the inhibition of FabI, LpxC would be stabilized Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.12 Escherichia coli P0AC75 W3110
-
2.4.99.12 Escherichia coli K12 P0AC75 W3110
-
2.7.1.130 Escherichia coli P27300 W3110
-
2.7.1.130 Escherichia coli K12 P27300 W3110
-
3.5.1.108 Escherichia coli P0A725 W3110
-
3.5.1.108 Escherichia coli K12 P0A725 W3110
-

Synonyms

EC Number Synonyms Comment Organism
2.4.99.12 WaaA
-
 Escherichia coli
2.7.1.130 LpxK
-
 Escherichia coli
3.5.1.108 LpxC
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
2.4.99.12 Escherichia coli addition of palmitic acid to the growth medium of wild-type Escherichia coli elevates the levels of LpxC by 1.7-fold up

General Information

EC Number General Information Comment Organism
2.4.99.12 metabolism a model for the biosynthesis of the outer membrane in Escherichia coli is presented. Lipopolysaccharide is an endotoxin that elicits a strong immune response from humans, and its biosynthesis is in part regulated via degradation of LpxC and WaaA enzymes by the protease FtsH. Overexpression of waaA results in increased levels of 3-deoxy-D-manno-oct-2-ulosonic acid sugar in membrane extracts. Kdo and heptose levels are not elevated in lipopolysaccharides. This implies that uncontrolled production of WaaA does not increase the lipopolysaccharide production rate but rather reglycosylates lipid A precursors Escherichia coli
2.7.1.130 metabolism a model for the biosynthesis of the outer membrane in Escherichia coli is presented. The catalytic activity is dependent on the concentration of unsaturated fatty acids. LpxC is additionally regulated by an unidentified protease whose activity is independent of lipid A disaccharide concentration (the feedback source for FtsH-mediated LpxC regulation) but can be induced in vitro by palmitic acid Escherichia coli
3.5.1.108 metabolism a model for the biosynthesis of the outer membrane in Escherichia coli is presented. Lipopolysaccharide is an endotoxin that elicits a strong immune response from humans, and its biosynthesis is in part regulated via degradation of LpxC and WaaA enzymes by the protease FtsH. Addition of palmitic acid to the growth medium of wild-type Escherichia coli elevates the levels of LpxC by 1.7fold. Palmitoyl-CoA induces a strong inhibitory effect on FabI. Due to the inhibition of FabI, LpxC would be stabilized. Thus, elevated cellular palmitoyl-CoA concentrations has an opposite effect on LpxC stability than the free-form of palmitic acid Escherichia coli