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Literature summary extracted from

  • Gangoiti, J.; Lamothe, L.; van Leeuwen, S.S.; Vafiadi, C.; Dijkhuizen, L.
    Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new glycoside hydrolase 70 subfamily of 4,6-alpha-glucanotransferase enzymes (2017), PLoS ONE, 12, e0172622 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B34 gene gtfD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His6-tagged full-length GtfD enzyme without its putative signal peptide-encoding sequence (amino acids 31 to 776) in Escherichia coli strain BL21 Star (DE3) Paenibacillus beijingensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B34 Ca2+ required Paenibacillus beijingensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B34 Paenibacillus beijingensis
-
-
-
2.4.1.B34 Paenibacillus beijingensis DSM 24997
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.B34 recombinant N-terminally His6-tagged GtfD enzyme without its putative signal peptide-encoding sequence (amino acids 31 to 776) from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography and ultrafiltration Paenibacillus beijingensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.B34 6.3
-
 purified recombinant enzyme, pH 7.0, 50°C, 1 mM CaCl2 Paenibacillus beijingensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B34 amylopectin + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + maltoheptaose transglycosylation activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + maltohexaose transglycosylation activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + maltopentaose transglycosylation activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + maltotetraose transglycosylation activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 amylose V + maltotriose transglycosylation activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 maltoheptaose + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 maltoheptaose + H2O hydrolytic activity Paenibacillus beijingensis DSM 24997 ?
-
 ?
2.4.1.B34 maltohexaose + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 maltohexaose + H2O hydrolytic activity Paenibacillus beijingensis DSM 24997 ?
-
 ?
2.4.1.B34 maltopentaose + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 maltopentaose + H2O hydrolytic activity Paenibacillus beijingensis DSM 24997 ?
-
 ?
2.4.1.B34 maltotetraose + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 maltotetraose + H2O hydrolytic activity Paenibacillus beijingensis DSM 24997 ?
-
 ?
2.4.1.B34 maltotriose + H2O hydrolytic activity Paenibacillus beijingensis ?
-
 ?
2.4.1.B34 additional information the enzyme GtfD shows both hydrolysis and transglycosylase (disproportionation) activity. 1HNMR analysis of the product mixture generated from amylose V reveals the presence of two broad anomeric signals corresponding to the (alpha1->4) and the newly formed (alpha1->6) linkages. Substrate specificity with maltooligosaccharides, overview. The Paenibacillus beijingensis GtfD enzyme is inactive on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5, while the enzyme catalyzes the conversion of malto-oligosaccharides (MOS) of DP3 to 7 showing both hydrolysis and transglycosylase (disproportionation) activity revealing the formation of lower- and higher-molecular-mass products Paenibacillus beijingensis ?
-
-
2.4.1.B34 additional information the enzyme GtfD shows both hydrolysis and transglycosylase (disproportionation) activity. 1HNMR analysis of the product mixture generated from amylose V reveals the presence of two broad anomeric signals corresponding to the (alpha1->4) and the newly formed (alpha1->6) linkages. Substrate specificity with maltooligosaccharides, overview. The Paenibacillus beijingensis GtfD enzyme is inactive on sucrose, panose, nigerose, and isomalto-oligosaccharides with DP2, DP3, and DP5, while the enzyme catalyzes the conversion of malto-oligosaccharides (MOS) of DP3 to 7 showing both hydrolysis and transglycosylase (disproportionation) activity revealing the formation of lower- and higher-molecular-mass products Paenibacillus beijingensis DSM 24997 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B34 GtfD
-
 Paenibacillus beijingensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B34 37
-
 assay at Paenibacillus beijingensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B34 7
-
 assay at Paenibacillus beijingensis