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Literature summary extracted from

  • Rennie, E.A.; Ebert, B.; Miles, G.P.; Cahoon, R.E.; Christiansen, K.M.; Stonebloom, S.; Khatab, H.; Twell, D.; Petzold, C.J.; Adams, P.D.; Dupree, P.; Heazlewood, J.L.; Cahoon, E.B.; Scheller, H.V.
    Identification of a sphingolipid alpha-glucuronosyltransferase that is essential for pollen function in Arabidopsis (2014), Plant Cell, 26, 3314-3325 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 gene IPUT1, functional enzyme overexpression in Saccharomyces cerevisiae, coexpression with a human UDP-GlcA transporter, GIPCs from Saccharomyces cerevisiae do not contain GlcA. Production of GlcA-IPC in yeast Arabidopsis thaliana
2.4.1.17 IPUT1 overexpression in Saccharomyces Nicotiana benthamiana using the Agrobacterium tumefaciens strain C58-1 transfection method, coexpression with a glucuronosyltransferase, GUX1. Total glucosylceramide levels decrease slightly compared with the p19 control, while levels of GlcN-GlcA-IPC increase Nicotiana benthamiana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.17 additional information introduction of Arabdiopsis thaliana IPUT1 gene together with genes for a UDP-glucose dehydrogenase from Arabidopsis thaliana and a human UDP-GlcA transporter into a yeast mutant deficient in the endogenous inositol phosphorylceramide (IPC) mannosyltransferase. In this engineered yeast strain, IPUT1 transfers GlcA to IPC Arabidopsis thaliana
2.4.1.17 additional information overexpression or silencing of IPUT1 in Nicotiana benthamiana results in an increase or a decrease, respectively, in IPC glucuronosyltransferase activity in vitro. Plants in which IPUT1 is silenced accumulate IPC, the immediate precursor, as well as ceramides and glucosylceramides. Plants overexpressing IPUT1 show an increased content of GIPCs. Mutations in IPUT1 are not transmitted through pollen, indicating that these sphingolipids are essential in plants. Silencing of the Nicotiana benthamiana IPUT1 homologue results in a reduction of the [14C]GlcA-IPC band to about 35% of that produced by GUS-silenced control microsomes Nicotiana benthamiana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 membrane
-
 Nicotiana benthamiana 16020
-
2.4.1.17 membrane
-
 Arabidopsis thaliana 16020
-
2.4.1.17 microsome
-
 Nicotiana benthamiana
-
-
2.4.1.17 microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.17 UDP-glucuronate + acceptor Nicotiana benthamiana transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) UDP + acceptor beta-D-glucuronoside
-
 ?
2.4.1.17 UDP-glucuronate + acceptor Arabidopsis thaliana transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) UDP + acceptor beta-D-glucuronoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Arabidopsis thaliana Q8GWB7
-
-
2.4.1.17 Nicotiana benthamiana
-
 cv. Domin
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 pollen
-
 Nicotiana benthamiana
-
2.4.1.17 pollen
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 UDP-glucuronate + acceptor transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana UDP + acceptor beta-D-glucuronoside
-
 ?
2.4.1.17 UDP-glucuronate + acceptor transfer of glucuronic acid (GlcA) from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana UDP + acceptor beta-D-glucuronoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 inositol phosphorylceramide glucuronosyltransferase1
-
 Nicotiana benthamiana
2.4.1.17 inositol phosphorylceramide glucuronosyltransferase1
-
 Arabidopsis thaliana
2.4.1.17 IPUT1
-
 Nicotiana benthamiana
2.4.1.17 IPUT1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 21
-
 assay at Nicotiana benthamiana
2.4.1.17 21
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7
-
 assay at Nicotiana benthamiana
2.4.1.17 7
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.4.1.17 evolution IPUT1 belongs to a glycosyltransferase family that transfers sugars with a retaining mechanism resulting in alpha-linked sugars, consistent with the alpha conformation of the GlcA in glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana
2.4.1.17 evolution IPUT1 belongs to a glycosyltransferase family that transfers sugars with a retaining mechanism resulting in alpha-linked sugars, consistent with the alpha conformation of the GlcA in glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana
2.4.1.17 malfunction overexpression or silencing of IPUT1 in Nicotiana benthamiana results in an increase or a decrease, respectively, in IPC glucuronosyltransferase activity in vitro. Plants in which IPUT1 is silenced accumulate IPC, the immediate precursor, as well as ceramides and glucosylceramides. Plants overexpressing IPUT1 show an increased content of GIPCs. Mutations in IPUT1 are not transmitted through pollen, indicating that these sphingolipids are essential in plants Nicotiana benthamiana
2.4.1.17 metabolism the enzyme is involved in the plant-type sphingolipid pathway Nicotiana benthamiana
2.4.1.17 metabolism the enzyme is involved in the plant-type sphingolipid pathway Arabidopsis thaliana
2.4.1.17 physiological function inositol phosphorylceramide glucuronosyltransferase1 (IPUT1) transfers GlcA from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Nicotiana benthamiana
2.4.1.17 physiological function inositol phosphorylceramide glucuronosyltransferase1 (IPUT1) transfers GlcA from UDP-GlcA to glycosyl inositol phosphorylceramides (GIPCs) Arabidopsis thaliana