EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.52 | gene serC, recombinant overexpression in Escherichia coli strain AB2829 harbouring pKD501 plasmid | Escherichia coli |
2.6.1.52 | gene serC, recombinant overexpression in Escherichia coli strain BL21(DE3) | Alkalihalobacillus alcalophilus |
2.6.1.52 | gene serC, recombinant overexpression of enzyme mutant K2G in Escherichia coli strain XL-1 Blue | Niallia circulans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.52 | K2G | site-directed mutagenesis | Niallia circulans |
2.6.1.52 | additional information | mutant C2-A' | Tetradesmus obliquus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.52 | additional information | - |
additional information | enzyme kinetics, overview | Niallia circulans | |
2.6.1.52 | additional information | - |
additional information | enzyme kinetics, overview | Alkalihalobacillus alcalophilus | |
2.6.1.52 | additional information | - |
additional information | enzyme kinetics, overview | Escherichia coli | |
2.6.1.52 | 0.0017 | - |
3-phosphooxypyruvate | pH 8.2, 25°C, recombinant enzyme mutant K2G | Niallia circulans | |
2.6.1.52 | 0.004 | - |
3-phosphooxypyruvate | pH 8.2, 25°C, recombinant enzyme | Escherichia coli | |
2.6.1.52 | 0.005 | - |
3-phosphooxypyruvate | pH 8.2, 25°C, recombinant enzyme | Bos taurus | |
2.6.1.52 | 0.0173 | - |
3-phosphooxypyruvate | pH 9.5, 25°C, recombinant enzyme mutant K2G | Niallia circulans | |
2.6.1.52 | 0.06 | - |
3-phosphooxypyruvate | pH 7.5-9.0, 25°C, recombinant enzyme | Glycine max | |
2.6.1.52 | 0.25 | - |
3-phosphooxypyruvate | pH 8.2, 25°C, recombinant enzyme | Ovis aries | |
2.6.1.52 | 0.5 | - |
L-glutamate | pH 7.5-9.0, 25°C, recombinant enzyme | Glycine max | |
2.6.1.52 | 0.8 | - |
L-glutamate | pH 9.5, 25°C, recombinant enzyme mutant K2G | Niallia circulans | |
2.6.1.52 | 1.2 | - |
L-glutamate | pH 8.2, 25°C, recombinant enzyme | Escherichia coli | |
2.6.1.52 | 1.2 | - |
L-glutamate | pH 8.2, 25°C, recombinant enzyme | Bos taurus | |
2.6.1.52 | 1.4 | - |
L-glutamate | pH 8.2, 25°C, recombinant enzyme mutant K2G | Niallia circulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 66000 | - |
recombinant enzyme K2G, gel filtration | Niallia circulans |
2.6.1.52 | 68000 | - |
gel filtration | Escherichia coli |
2.6.1.52 | 77000 | - |
gel filtration | Bos taurus |
2.6.1.52 | 79800 | 81200 | - |
Escherichia coli |
2.6.1.52 | 80000 | - |
gel filtration | Tetradesmus obliquus |
2.6.1.52 | 85000 | - |
gel filtration | Glycine max |
2.6.1.52 | 90700 | - |
sedimentation equilibrium | Bos taurus |
2.6.1.52 | 96000 | - |
sedimentation equilibrium | Ovis aries |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Niallia circulans | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Alkalihalobacillus alcalophilus | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Escherichia coli | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Bos taurus | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Ovis aries | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Tetradesmus obliquus | - |
3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | Glycine max | - |
3-phosphooxypyruvate + L-glutamate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.52 | Alkalihalobacillus alcalophilus | Q9RME2 | i.e. Bacillus alcalophilus | - |
2.6.1.52 | Bos taurus | A0A3S5ZPF7 | - |
- |
2.6.1.52 | Escherichia coli | E2QJB8 | - |
- |
2.6.1.52 | Escherichia coli | P23721 | - |
- |
2.6.1.52 | Glycine max | I1JCA9 | - |
- |
2.6.1.52 | Niallia circulans | Q59196 | var. alcalophilus | - |
2.6.1.52 | Ovis aries | W5PS11 | - |
- |
2.6.1.52 | Tetradesmus obliquus | Q9S8N7 | i.e. Acutodesmus obliquus | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.52 | recombinant enzyme 15.6fold from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, ultrafiltration, and gel filtration | Alkalihalobacillus alcalophilus |
2.6.1.52 | recombinant enzyme mutant K2G 5.5fold from Escherichia coli strain XL-1 Blue by anion exchange chromatography, ultrafiltration, and gel filtration | Niallia circulans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.52 | brain | - |
Ovis aries | - |
2.6.1.52 | liver | - |
Bos taurus | - |
2.6.1.52 | additional information | the alkaliphilic microbe grows optimally at pH 9.5-10.5 | Niallia circulans | - |
2.6.1.52 | root nodule | - |
Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 1.3 | - |
purified enzyme, pH 8.1-8.2, 25°C | Ovis aries |
2.6.1.52 | 3.8 | - |
pH not specified in the publication, 25°C, purified recombinant enzyme, substrate 3-phosphooxypyruvate | Escherichia coli |
2.6.1.52 | 7 | - |
purified enzyme, pH and temperature not specified in the publication | Glycine max |
2.6.1.52 | 42 | - |
purified recombinant enzyme, pH 6.1, 25°C | Alkalihalobacillus alcalophilus |
2.6.1.52 | 42.3 | - |
purified recombinant enzyme, pH 8.2, 25°C | Escherichia coli |
2.6.1.52 | 44.4 | - |
purified recombinant enzyme mutant K2G, pH 6.1, 25°C | Niallia circulans |
2.6.1.52 | 1416 | - |
purified enzyme, pH 6.8-7.2, 38°C | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Niallia circulans | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Alkalihalobacillus alcalophilus | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Escherichia coli | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Bos taurus | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Ovis aries | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Tetradesmus obliquus | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | 3-phosphooxypyruvate + L-glutamate | - |
Glycine max | O-phospho-L-serine + 2-oxoglutarate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Niallia circulans | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Alkalihalobacillus alcalophilus | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Escherichia coli | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Bos taurus | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Ovis aries | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Tetradesmus obliquus | 3-phosphooxypyruvate + L-glutamate | - |
r | |
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Glycine max | 3-phosphooxypyruvate + L-glutamate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.52 | ? | x * 39500-40500, recombinant enzyme, SDS-PAGE, x * 40200, about, sequence calculation | Alkalihalobacillus alcalophilus |
2.6.1.52 | homodimer | 2 * 40000, SDS-PAGE | Tetradesmus obliquus |
2.6.1.52 | homodimer | 2 * 43000, SDS-PAGE | Bos taurus |
2.6.1.52 | homodimer | 2 * 38000-39800, recombinant enzyme mutant K2G, SDS-PAGE, 2 * 39793, sequence calculation | Niallia circulans |
2.6.1.52 | homodimer | 2 * 39000-40000, SDS-PAGE, 2 * 39834, sequence calculation | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.52 | phosphoserine aminotransferases | - |
Niallia circulans |
2.6.1.52 | phosphoserine aminotransferases | - |
Alkalihalobacillus alcalophilus |
2.6.1.52 | phosphoserine aminotransferases | - |
Escherichia coli |
2.6.1.52 | phosphoserine aminotransferases | - |
Bos taurus |
2.6.1.52 | phosphoserine aminotransferases | - |
Ovis aries |
2.6.1.52 | phosphoserine aminotransferases | - |
Tetradesmus obliquus |
2.6.1.52 | phosphoserine aminotransferases | - |
Glycine max |
2.6.1.52 | PSAT | - |
Niallia circulans |
2.6.1.52 | PSAT | - |
Alkalihalobacillus alcalophilus |
2.6.1.52 | PSAT | - |
Escherichia coli |
2.6.1.52 | PSAT | - |
Bos taurus |
2.6.1.52 | PSAT | - |
Ovis aries |
2.6.1.52 | PSAT | - |
Tetradesmus obliquus |
2.6.1.52 | PSAT | - |
Glycine max |
2.6.1.52 | PSAT-BALC | - |
Alkalihalobacillus alcalophilus |
2.6.1.52 | PSAT-BCIRA | - |
Niallia circulans |
2.6.1.52 | PSAT-ECOLI | - |
Escherichia coli |
2.6.1.52 | PSAT1 | - |
Bos taurus |
2.6.1.52 | serC | - |
Niallia circulans |
2.6.1.52 | serC | - |
Alkalihalobacillus alcalophilus |
2.6.1.52 | serC | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 25 | - |
assay at | Niallia circulans |
2.6.1.52 | 25 | - |
assay at | Alkalihalobacillus alcalophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 6.8 | 7.2 | - |
Bos taurus |
2.6.1.52 | 6.8 | 8.2 | - |
Tetradesmus obliquus |
2.6.1.52 | 7.5 | 9 | broad optimum | Glycine max |
2.6.1.52 | 7.5 | 8.5 | - |
Escherichia coli |
2.6.1.52 | 8.1 | 8.2 | - |
Ovis aries |
2.6.1.52 | 9.4 | - |
- |
Alkalihalobacillus alcalophilus |
2.6.1.52 | 9.5 | - |
- |
Niallia circulans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 6.5 | 12 | activity range, profile overview | Niallia circulans |
2.6.1.52 | 6.5 | 12 | activity range, profile overview | Alkalihalobacillus alcalophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Niallia circulans | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Alkalihalobacillus alcalophilus | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Escherichia coli | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Bos taurus | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Ovis aries | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Tetradesmus obliquus | |
2.6.1.52 | pyridoxal 5'-phosphate | PLP, dependent on | Glycine max |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.6.1.52 | Niallia circulans | isoelectric focusing | - |
4.6 |
2.6.1.52 | Alkalihalobacillus alcalophilus | isoelectric focusing | - |
4.6 |
2.6.1.52 | Escherichia coli | isoelectric focusing | - |
4.6 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Niallia circulans |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Alkalihalobacillus alcalophilus |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Escherichia coli |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Bos taurus |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Ovis aries |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Tetradesmus obliquus |
2.6.1.52 | metabolism | enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis | Glycine max |