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Literature summary extracted from

  • Meinsma, R.; van Kuilenburg, A.B.
    Purification, activity, and expression levels of two uridine-cytidine kinase isoforms in neuroblastoma cell lines (2016), Nucleosides Nucleotides Nucleic Acids, 35, 613-618 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.48 gene UCK1, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) Homo sapiens
2.7.1.48 gene UCK2, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 additional information
-
 additional information steady-state kinetics Homo sapiens
2.7.1.48 0.1
-
 cytidine recombinant isozyme UCK2, pH 7.6, 37°C Homo sapiens
2.7.1.48 0.13
-
 uridine recombinant isozyme UCK2, pH 7.6, 37°C Homo sapiens
2.7.1.48 4
-
 cytidine recombinant isozyme UCK1, pH 7.6, 37°C Homo sapiens
2.7.1.48 5.1
-
 uridine recombinant isozyme UCK1, pH 7.6, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.48 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.48 ATP + cytidine Homo sapiens
-
 ADP + CMP
-
 ?
2.7.1.48 ATP + uridine Homo sapiens
-
 ADP + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Homo sapiens Q9BZX2
-
-
2.7.1.48 Homo sapiens Q9HA47
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens
2.7.1.48 recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.48 FISK cell
-
 Homo sapiens
-
2.7.1.48 IMR-32 cell
-
 Homo sapiens
-
2.7.1.48 NASS cell
-
 Homo sapiens
-
2.7.1.48 neuroblastoma cell generation of a specific antibody against UCK1 and analysis of expression of both UCK isoforms 1 and 2 in a panel of neuroblastoma cell lines, overview. No differences are observed for UCK1 and UCK2 expression between MYCN-single copy cell lines and MYCN-amplified neuroblastoma cell lines Homo sapiens
-
2.7.1.48 SH-SY5Y cell
-
 Homo sapiens
-
2.7.1.48 SJNB-10 cell
-
 Homo sapiens
-
2.7.1.48 SJNB-8 cell
-
 Homo sapiens
-
2.7.1.48 SK-N-BE(2) cell
-
 Homo sapiens
-
2.7.1.48 SK-N-SH cell
-
 Homo sapiens
-
2.7.1.48 Tet21N cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + cytidine
-
 Homo sapiens ADP + CMP
-
 ?
2.7.1.48 ATP + uridine
-
 Homo sapiens ADP + UMP
-
 ?
2.7.1.48 additional information uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine, cytidine, and several pyrimidine ribonucleoside analogues Homo sapiens ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.48 ? x * 29000, recombinant isozyme UCK2, SDS-PAGE Homo sapiens
2.7.1.48 ? x * 31000, recombinant isozyme UCK1, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.48 UCK1
-
 Homo sapiens
2.7.1.48 UCK2
-
 Homo sapiens
2.7.1.48 uridine-cytidine kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.48 7.6
-
 assay at Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.48 0.59
-
 uridine recombinant isozyme UCK1, pH 7.6, 37°C Homo sapiens
2.7.1.48 0.69
-
 cytidine recombinant isozyme UCK1, pH 7.6, 37°C Homo sapiens
2.7.1.48 210
-
 cytidine recombinant isozyme UCK2, pH 7.6, 37°C Homo sapiens
2.7.1.48 470
-
 uridine recombinant isozyme UCK2, pH 7.6, 37°C Homo sapiens