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Literature summary extracted from

  • Diretto, G.; Ahrazem, O.; Rubio-Moraga, A.; Fiore, A.; Sevi, F.; Argandona, J.; Gomez-Gomez, L.
    UGT709G1 a novel uridine diphosphate glycosyltransferase involved in the biosynthesis of picrocrocin, the precursor of safranal in saffron (Crocus sativus) (2019), New Phytol., 224, 725-740 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B83 expression in Escherichia coli Crocus sativus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.B83 homology modeling of structure. The predicted substrate tunnel is formed by the amino acid residues P16, G17, Q18, G19, H20, R45, F82, L89, F201, L290, H359, G361, F381, A382, and Q384 Crocus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.B83 0.064
-
 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene pH 8.0, 30°C Crocus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.B83 cytosol
-
 Crocus sativus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B83 UDP-D-glucose + 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene Crocus sativus
-
 UDP + picrocrocin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B83 Crocus sativus A0A343AXU3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.B83 additional information no expression is detected in leaves, corms, or roots Crocus sativus
-
2.4.1.B83 stigma expression of UGT709G1 occurs preferentially in the earlier developmental stages, with low transcripts levels also detected in fully developed stigmas Crocus sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B83 additional information no substrates: crocetin, quercetin, kaempferol, dihydrokaempferol, rhamnetin, isorhamnetin, lutein, apigenin, myricetin, caffeic acid, coumaric acid, genistein, esculetin, trans-cinnamic acid, sinapic acid, indole acetic acid, and abscisic acid Crocus sativus ?
-
-
2.4.1.B83 UDP-D-glucose + 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene
-
 Crocus sativus UDP + picrocrocin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B83 ? x * 57500, calculated from sequence Crocus sativus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B83 UGT709G1
-
 Crocus sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.B83 0.0074
-
 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene pH 8.0, 30°C Crocus sativus

General Information

EC Number General Information Comment Organism
2.4.1.B83 physiological function coexpression of CCD2L and UGT709G1 in Nicothiana benthamiana leads to interception of carotenoid flux to cleave zeaxanthin and to produce picrocrocin Crocus sativus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.B83 0.11
-
 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene pH 8.0, 30°C Crocus sativus