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Literature summary extracted from

  • Silva, R.; Aguiar, T.Q.; Oliveira, C.; Domingues, L.
    Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii (2019), New Biotechnol., 50, 1-8 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 gene AGOS_AFR052C, sequence comparisons, phylogenetic tree, functional recombinant expression of the enzyme with an N-terminal fusion to TrxA as a soluble enzyme in Escherichia coli Eremothecium gossypii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.9 additional information the pyrimidine auxotroph Ashbya gossypii mutant Agura3 shows hypersensitivity to uracil. Uridine is the only intermediate of the pyrimidine salvage pathway able to fully restore this mutant's growth. Overexpression of the AgUPRT encoding-gene in Ashbya gossypii mutant Agura3 leads to similar growth on rich medium containing 5 mM uracil or uridine Eremothecium gossypii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 Uracil concentrations above 1mM negatively affect UPRT activity Eremothecium gossypii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 UMP + diphosphate Eremothecium gossypii
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate Eremothecium gossypii CBS 109.51
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate Eremothecium gossypii FGSC 9923
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate Eremothecium gossypii ATCC 10895
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate Eremothecium gossypii NRRL Y-1056
-
 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Eremothecium gossypii Q754M0 i.e. Eremothecium gossypii
-
2.4.2.9 Eremothecium gossypii ATCC 10895 Q754M0 i.e. Eremothecium gossypii
-
2.4.2.9 Eremothecium gossypii CBS 109.51 Q754M0 i.e. Eremothecium gossypii
-
2.4.2.9 Eremothecium gossypii FGSC 9923 Q754M0 i.e. Eremothecium gossypii
-
2.4.2.9 Eremothecium gossypii NRRL Y-1056 Q754M0 i.e. Eremothecium gossypii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 recombinant enzyme from Escherichia coli, cleavage of the TrxA fusion protein with TEV protease Eremothecium gossypii

Storage Stability

EC Number Storage Stability Organism
2.4.2.9 4°C, purified detagged recombinant enzyme, in Tris-NaCl buffer, stable for at least 1 week Eremothecium gossypii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 UMP + diphosphate
-
 Eremothecium gossypii uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter Eremothecium gossypii uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate
-
 Eremothecium gossypii CBS 109.51 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter Eremothecium gossypii CBS 109.51 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate
-
 Eremothecium gossypii FGSC 9923 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter Eremothecium gossypii FGSC 9923 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate
-
 Eremothecium gossypii ATCC 10895 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter Eremothecium gossypii ATCC 10895 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate
-
 Eremothecium gossypii NRRL Y-1056 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.9 UMP + diphosphate maximum activity is obtained with 0.4-0.5 mM uracil in the presence of 1 mM PRPP, decreasing with increasing uracil concentrations thereafter. The activity in the presence of 0.5 mM uracil and at varying concentrations of PRPP is maximal at 0.75-1.0 mM PRPP, remaining constant thereafter Eremothecium gossypii NRRL Y-1056 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 ? x * 26000, recombinant detagged enzyme, SDS-PAGE Eremothecium gossypii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 AFR052C
-
 Eremothecium gossypii
2.4.2.9 AFR052Cp UniProt Eremothecium gossypii
2.4.2.9 AGOS_AFR052C locus name Eremothecium gossypii
2.4.2.9 AgUPRT
-
 Eremothecium gossypii
2.4.2.9 UPRT
-
 Eremothecium gossypii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.9 30
-
 assay at Eremothecium gossypii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 7.5
-
 assay at Eremothecium gossypii

General Information

EC Number General Information Comment Organism
2.4.2.9 evolution UPRT is an enzyme that is highly conserved across species and has been characterized in several organisms, from prokaryotes to human Eremothecium gossypii
2.4.2.9 malfunction the pyrimidine auxotroph Ashbya gossypii mutant Agura3 shows hypersensitivity to uracil. The mutant's growth can be restored by uridine. In silico analysis of the AgUPRT does not highlight any particular feature that can account for limitations in its enzymatic activity Eremothecium gossypii
2.4.2.9 physiological function uracil phosphoribosyltransferase (UPRT) is the pyrimidine salvage pathway enzyme responsible for converting uracil to uridine monophosphate in the presence of phosphoribosyl pyrophosphate (PRPP). Uracil-mediated regulation mechanism of the UPRT activity Eremothecium gossypii