Literature summary extracted from

Hsu, T.M.; Welner, D.H.; Russ, Z.N.; Cervantes, B.; Prathuri, R.L.; Adams, P.D.; Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy (2018), Nat. Chem. Biol., 14, 256-261 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.220	heterologous expression of PtUGT1 in Escherichia coli	Persicaria tinctoria

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.4.1.220	the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site	Persicaria tinctoria

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.220	Persicaria tinctoria	-	-	-
2.4.1.220	Persicaria tinctoria	A0A2R2JFJ4	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.220	-	Persicaria tinctoria

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.220	UDP-glucose + indoxyl	-	Persicaria tinctoria	UDP + indican	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.220	PtUGT1	predominant isoform	Persicaria tinctoria
2.4.1.220	PtUGT2	differs from PtUGT1 by four amino acid residues V19M, G225A, E230Q, and A423D	Persicaria tinctoria
2.4.1.220	UDP-glucose:indoxyl glucosyltransferase	-	Persicaria tinctoria
2.4.1.220	UGIG	-	Persicaria tinctoria
2.4.1.220	UGT72B29	-	Persicaria tinctoria
2.4.1.220	UGT72B30	-	Persicaria tinctoria

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4.1.220	9.1	-	UDP-glucose	pH 7.8, 30°C	Persicaria tinctoria
2.4.1.220	12	-	UDP-glucose	pH 7.8, 30°C	Persicaria tinctoria

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Release 2023.1 (January 2023)