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Literature summary extracted from

  • Barja, M.V.; Rodriguez-Concepcion, M.
    A Simple In vitro assay to measure the activity of geranylgeranyl diphosphate synthase and other short-chain prenyltransferases (2020), Methods Mol. Biol., 2083, 27-38 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.1 chloroplast
-
 Arabidopsis thaliana 9507
-
2.5.1.10 chloroplast
-
 Arabidopsis thaliana 9507
-
2.5.1.29 chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Arabidopsis thaliana
-
 diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.1 Arabidopsis thaliana P34802
-
-
2.5.1.10 Arabidopsis thaliana P34802
-
-
2.5.1.29 Arabidopsis thaliana P34802
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.1
-
 Arabidopsis thaliana
2.5.1.10
-
 Arabidopsis thaliana
2.5.1.29
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.1 additional information
-
 a fast and inexpensive spectrophotometric protocol is described for determining the kinetic parameters of SC-PTs in purified enzyme preparations, using an adapted assay for diphosphate quantification Arabidopsis thaliana
2.5.1.10 additional information
-
 a fast and inexpensive spectrophotometric protocol is described for determining the kinetic parameters of SC-PTs in purified enzyme preparations, using an adapted assay for diphosphate quantification Arabidopsis thaliana
2.5.1.29 additional information
-
 a fast and inexpensive spectrophotometric protocol is described for determining the kinetic parameters of SC-PTs in purified enzyme preparations, using an adapted assay for diphosphate quantification Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.1 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.1 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranyl diphosphate
-
 ?
2.5.1.1 geranyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.10, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.29, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Arabidopsis thaliana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + geranyl diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.10, the enzyme catalyze the sequential head-to-tail addition of three C5 molecules of isopentenyl diphosphate to dimethylallyl diphosphate with the concomitant release of pyrophosphate Arabidopsis thaliana diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.1 At4g36810
-
 Arabidopsis thaliana
2.5.1.1 GGPPS11
-
 Arabidopsis thaliana
2.5.1.10 At4g36810
-
 Arabidopsis thaliana
2.5.1.10 GGPPS11
-
 Arabidopsis thaliana
2.5.1.29 At4g36810
-
 Arabidopsis thaliana
2.5.1.29 GGPPS11
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 7.5
-
 assay at. A simple in vitro assay is developed to measure the activity of geranylgeranyl diphosphate synthase and other short-chain prenyltransferases Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.5.1.1 metabolism produces geranylgeranyl diphosphate for the synthesis of carotenoids in the chloroplast Arabidopsis thaliana
2.5.1.10 metabolism produces geranylgeranyl diphosphate for the synthesis of carotenoids in the chloroplast Arabidopsis thaliana
2.5.1.29 metabolism produces geranylgeranyl diphosphate for the synthesis of carotenoids in the chloroplast Arabidopsis thaliana
2.5.1.29 additional information the enzyme supplies geranylgeranyl diphosphate or the production of carotenoids and other groups of plastidial isoprenoids Arabidopsis thaliana