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Literature summary extracted from

  • Harun-Ur-Rashid, M.; Oogai, S.; Parveen, S.; Inafuku, M.; Iwasaki, H.; Fukuta, M.; Amzad Hossain, M.; Oku, H.
    Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala (2020), J. Plant Res., 133, 95-108 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.47 molecular docking of substrates Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.06
-
 hydrogen sulfide pH 7.0, 40°C Leucaena leucocephala
2.5.1.47 0.078
-
 hydrogen sulfide pH 7.5, 23°C Leucaena leucocephala
2.5.1.47 0.148
-
 O-acetyl-L-Ser pH 7.5, 23°C Leucaena leucocephala
2.5.1.47 0.838
-
 O-acetyl-L-Ser pH 7.0, 40°C Leucaena leucocephala

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 chloroplast
-
 Leucaena leucocephala 9507
-
2.5.1.47 cytosol
-
 Leucaena leucocephala 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Leucaena leucocephala
-
-
-
2.5.1.47 Leucaena leucocephala A0A2Z6G0Q7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information enzyme additionally catalyzes synthesis of mimosine, reaction of EC 2.5.1.52. The apparent kcat for Cys production is over sixfold higher than mimosine synthesis and the apparent Km is 3.7 times lower Leucaena leucocephala ?
-
-
2.5.1.47 additional information enzyme is unable to synthesize mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala ?
-
-
2.5.1.47 O-acetyl-L-Ser + hydrogen sulfide
-
 Leucaena leucocephala L-Cys + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 ? x * 34290, calculated from sequence Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 35
-
 synthesis of mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala
2.5.1.47 40
-
-
 Leucaena leucocephala
2.5.1.47 40
-
 cysteine synthesis Leucaena leucocephala

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 2.43
-
 hydrogen sulfide pH 7.0, 40°C Leucaena leucocephala
2.5.1.47 32.65
-
 hydrogen sulfide pH 7.5, 23°C Leucaena leucocephala
2.5.1.47 43.52
-
 O-acetyl-L-Ser pH 7.5, 23°C Leucaena leucocephala
2.5.1.47 72.83
-
 O-acetyl-L-Ser pH 7.0, 40°C Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7
-
-
 Leucaena leucocephala
2.5.1.47 7.5
-
 cysteine synthesis Leucaena leucocephala
2.5.1.47 8
-
 synthesis of mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.47 5.5 7.5
-
 Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Leucaena leucocephala

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.47 Leucaena leucocephala calculated from sequence
-
 8.7