Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jeong, W.S.; Lee, Y.R.; Hong, S.J.; Choi, S.J.; Choi, J.H.; Park, S.Y.; Woo, E.J.; Kim, Y.M.; Park, B.R.
    Carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides (2019), J. Microbiol. Biotechnol., 29, 1938-1946 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.248 the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified Thermoanaerobacter thermocopriae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.248 115000
-
 SDS-PAGE, the C-terminal region of TtCITase (Met740-Phe1559) Thermoanaerobacter thermocopriae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.248 Thermoanaerobacter thermocopriae WP_028992696
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.248 the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified Thermoanaerobacter thermocopriae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.248 isomaltopentaose the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates Thermoanaerobacter thermocopriae ?
-
 ?
2.4.1.248 maltodextrin the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates Thermoanaerobacter thermocopriae ?
-
 ?
2.4.1.248 maltopentaose the carboxy-terminal region of a thermostable CITase from Thermoanaerobacter thermocopriae has the ability to produce long isomaltooligosaccharides. It synthesizes alpha-1,6 glucosyl products with over seven degrees of polymerization by an alpha-1,6 glucosyl transfer reaction from maltopentaose, isomaltopentaose, or commercialized maltodextrins as substrates Thermoanaerobacter thermocopriae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.248 CITase
-
 Thermoanaerobacter thermocopriae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.248 40
-
-
 Thermoanaerobacter thermocopriae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.248 30 50 30°C: about 80% of maximal activity, 50°C: about 50% of maximal activity Thermoanaerobacter thermocopriae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.248 5.5
-
-
 Thermoanaerobacter thermocopriae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.248 4.5 6 pH 4.5: about 60% of maximal activity, pH 6.0: about 50% of maximal activity Thermoanaerobacter thermocopriae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.248 5.5
-
 18 h, enzyme retains 100% activity Thermoanaerobacter thermocopriae