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Literature summary extracted from

  • Fox, K.; Prather, K.
    Production of d-Glyceric acid from d-Galacturonate in Escherichia coli (2020), J. Ind. Microbiol. Biotechnol., 47, 1075-1081 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.165 synthesis a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate represents an alternative for the production of D-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability, overview Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.203 expressed in Escherichia coli DH5alpha cells Pseudomonas syringae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.165 additional information development of a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables D-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways leads to the development of a DELTAgarKDELTAhyiDELTAglxKDELTAuxaC mutant strain that produces 4.8 g/l of D-glyceric acid from D-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produces similar yields and demonstrates that galactose or glycerol serve as possible carbon co-feeds for industrial production, method overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+ Pseudomonas syringae
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
2.7.1.165 ATP + D-glycerate Escherichia coli
-
 ADP + 2-phospho-(R)-glycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Pseudomonas syringae
-
-
-
2.7.1.165 Escherichia coli P23524
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+
-
 Pseudomonas syringae D-galactaro-1,5-lactone + NADH + H+
-
 ?
2.7.1.165 ATP + D-glycerate
-
 Escherichia coli ADP + 2-phospho-(R)-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.203 Udh
-
 Pseudomonas syringae
2.7.1.165 garK
-
 Escherichia coli
2.7.1.165 glycerate kinase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Pseudomonas syringae