Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bezsudnova, E.Y.; Dibrova, D.V.; Nikolaeva, A.Y.; Rakitina, T.V.; Popov, V.O.
    Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases (2018), J. Biotechnol., 271, 26-28 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.B21 gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys Haliangium ochraceum
2.6.1.42 gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys Methanococcus vannielii
2.6.1.42 gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys Thermomicrobium roseum
2.6.1.42 gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV protease cleavage site in Escherichia coli strain BL21(DE3)pLys Thermobaculum terrenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.B21 11.8
-
 (R)-1-phenylethylamine pH 9.0, 40°C, recombinant enzyme, with 3-methyl-2-oxovalerate Haliangium ochraceum
2.6.1.42 10.3
-
 (R)-1-phenylethylamine pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate Thermobaculum terrenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermomicrobium roseum
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermobaculum terrenum
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii OCM 148
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermomicrobium roseum P-2
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii JCM 13029
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii SB
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermomicrobium roseum ATCC 27502
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii ATCC 35089
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus vannielii DSM 1224
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermomicrobium roseum DSM 5159
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate Thermobaculum terrenum YNP1
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B21 Haliangium ochraceum D0LR31
-
-
2.6.1.B21 Haliangium ochraceum DSM 14365 D0LR31
-
-
2.6.1.B21 Haliangium ochraceum JCM 11303 D0LR31
-
-
2.6.1.B21 Haliangium ochraceum SMP-2 D0LR31
-
-
2.6.1.42 Methanococcus vannielii A6UP94
-
-
2.6.1.42 Methanococcus vannielii ATCC 35089 A6UP94
-
-
2.6.1.42 Methanococcus vannielii DSM 1224 A6UP94
-
-
2.6.1.42 Methanococcus vannielii JCM 13029 A6UP94
-
-
2.6.1.42 Methanococcus vannielii OCM 148 A6UP94
-
-
2.6.1.42 Methanococcus vannielii SB A6UP94
-
-
2.6.1.42 Thermobaculum terrenum D1CCW1
-
-
2.6.1.42 Thermobaculum terrenum YNP1 D1CCW1
-
-
2.6.1.42 Thermomicrobium roseum B9L0B7
-
-
2.6.1.42 Thermomicrobium roseum ATCC 27502 B9L0B7
-
-
2.6.1.42 Thermomicrobium roseum DSM 5159 B9L0B7
-
-
2.6.1.42 Thermomicrobium roseum P-2 B9L0B7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.B21 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity Haliangium ochraceum
2.6.1.42 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity Methanococcus vannielii
2.6.1.42 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity Thermomicrobium roseum
2.6.1.42 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, to homogeneity Thermobaculum terrenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.B21 0.007
-
 purified recombinant enzyme, pH 8.0, 40°C, substrates (R)-1-phenylethylamine and 3-methyl-3-oxovalerate Haliangium ochraceum
2.6.1.B21 0.1
-
 purified recombinant enzyme, pH 9.0, 40°C, substrates (R)-1-phenylethylamine and 3-methyl-3-oxovalerate Haliangium ochraceum
2.6.1.42 0.007
-
 purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate Methanococcus vannielii
2.6.1.42 0.012
-
 purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate Thermobaculum terrenum
2.6.1.42 0.012
-
 purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate Thermobaculum terrenum
2.6.1.42 0.033
-
 purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate Thermomicrobium roseum
2.6.1.42 0.035
-
 purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate Methanococcus vannielii
2.6.1.42 0.15
-
 purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate Thermomicrobium roseum
2.6.1.42 3
-
 purified recombinant enzyme, pH 8.0, 50°C, substrates L-leucine and 2-oxoglutarate Methanococcus vannielii
2.6.1.42 37
-
 purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate Thermomicrobium roseum
2.6.1.42 42
-
 purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate Thermobaculum terrenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B21 (R)-1-phenylethylamine + 3-methyl-2-oxovalerate the enzyme is active towards (R)-PEA and not active towards (S)-PEA Haliangium ochraceum acetophenone + L-isoleucine
-
 r
2.6.1.B21 (R)-1-phenylethylamine + 3-methyl-2-oxovalerate the enzyme is active towards (R)-PEA and not active towards (S)-PEA Haliangium ochraceum JCM 11303 acetophenone + L-isoleucine
-
 r
2.6.1.B21 (R)-1-phenylethylamine + 3-methyl-2-oxovalerate the enzyme is active towards (R)-PEA and not active towards (S)-PEA Haliangium ochraceum DSM 14365 acetophenone + L-isoleucine
-
 r
2.6.1.B21 (R)-1-phenylethylamine + 3-methyl-2-oxovalerate the enzyme is active towards (R)-PEA and not active towards (S)-PEA Haliangium ochraceum SMP-2 acetophenone + L-isoleucine
-
 r
2.6.1.B21 additional information no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms Haliangium ochraceum ?
-
-
2.6.1.B21 additional information no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms Haliangium ochraceum JCM 11303 ?
-
-
2.6.1.B21 additional information no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms Haliangium ochraceum DSM 14365 ?
-
-
2.6.1.B21 additional information no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms Haliangium ochraceum SMP-2 ?
-
-
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermomicrobium roseum acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermobaculum terrenum acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii OCM 148 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermomicrobium roseum P-2 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii JCM 13029 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii SB acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermomicrobium roseum ATCC 27502 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii ATCC 35089 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Methanococcus vannielii DSM 1224 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermomicrobium roseum DSM 5159 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + 2-oxoglutarate
-
 Thermobaculum terrenum YNP1 acetophenone + L-glutamate
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermomicrobium roseum acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermobaculum terrenum acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii OCM 148 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermomicrobium roseum P-2 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii JCM 13029 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii SB acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermomicrobium roseum ATCC 27502 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii ATCC 35089 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Methanococcus vannielii DSM 1224 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermomicrobium roseum DSM 5159 acetophenone + L-alanine
-
 r
2.6.1.42 (R)-1-phenylethylamine + pyruvate (R)-PEA Thermobaculum terrenum YNP1 acetophenone + L-alanine
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermomicrobium roseum 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermobaculum terrenum 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii OCM 148 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermomicrobium roseum P-2 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii JCM 13029 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii SB 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermomicrobium roseum ATCC 27502 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii ATCC 35089 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Methanococcus vannielii DSM 1224 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermomicrobium roseum DSM 5159 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Thermobaculum terrenum YNP1 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermomicrobium roseum ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermobaculum terrenum ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii OCM 148 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermomicrobium roseum P-2 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii JCM 13029 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii SB ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermomicrobium roseum ATCC 27502 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii ATCC 35089 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Methanococcus vannielii DSM 1224 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermomicrobium roseum DSM 5159 ?
-
-
2.6.1.42 additional information substrate specificity, overview. The enzyme is active with (S)-amine substrates, but also with (R)-amines to a lower extent. No activity with beta-Ala and D-Ala. The enzyme is active towards (R)-PEA and not active towards (S)-PEA. Reaction mechanisms Thermobaculum terrenum YNP1 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B21 (R)-selective (R)-amine:pyruvate transaminase
-
 Haliangium ochraceum
2.6.1.B21 Hoch3033
-
 Haliangium ochraceum
2.6.1.B21 More cf. EC 2.6.1.42 Haliangium ochraceum
2.6.1.42 BcaT
-
 Methanococcus vannielii
2.6.1.42 BcaT
-
 Thermomicrobium roseum
2.6.1.42 BcaT
-
 Thermobaculum terrenum
2.6.1.42 branched-chain amino acid aminotransferase
-
 Methanococcus vannielii
2.6.1.42 branched-chain amino acid aminotransferase
-
 Thermomicrobium roseum
2.6.1.42 branched-chain amino acid aminotransferase
-
 Thermobaculum terrenum
2.6.1.42 IlvE
-
 Methanococcus vannielii
2.6.1.42 IlvE
-
 Thermomicrobium roseum
2.6.1.42 IlvE
-
 Thermobaculum terrenum
2.6.1.42 Mevan_0408
-
 Methanococcus vannielii
2.6.1.42 trd_1610
-
 Thermomicrobium roseum
2.6.1.42 Tter_1720
-
 Thermobaculum terrenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.B21 40
-
-
 Haliangium ochraceum
2.6.1.42 40
-
-
 Thermomicrobium roseum
2.6.1.42 40
-
-
 Thermobaculum terrenum
2.6.1.42 50
-
-
 Methanococcus vannielii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.B21 0.19
-
 (R)-1-phenylethylamine pH 9.0, 40°C, recombinant enzyme, with 3-methyl-2-oxovalerate Haliangium ochraceum
2.6.1.42 0.2
-
 (R)-1-phenylethylamine pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate Thermobaculum terrenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B21 9
-
 (R)-transamination with (R)-phenylethylamine Haliangium ochraceum
2.6.1.42 8
-
 (S)-transamination with L-leucine Methanococcus vannielii
2.6.1.42 8
-
 (S)-transamination with L-leucine Thermomicrobium roseum
2.6.1.42 8
-
 (S)-transamination with L-leucine Thermobaculum terrenum
2.6.1.42 9
-
 (R)-transamination with (R)-phenylethylamine Methanococcus vannielii
2.6.1.42 9
-
 (R)-transamination with (R)-phenylethylamine Thermomicrobium roseum
2.6.1.42 9
-
 (R)-transamination with (R)-phenylethylamine Thermobaculum terrenum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B21 pyridoxal 5'-phosphate PLP Haliangium ochraceum
2.6.1.42 pyridoxal 5'-phosphate PLP Methanococcus vannielii
2.6.1.42 pyridoxal 5'-phosphate PLP Thermomicrobium roseum
2.6.1.42 pyridoxal 5'-phosphate PLP Thermobaculum terrenum

General Information

EC Number General Information Comment Organism
2.6.1.B21 evolution the enzyme belongs to the PLP fold type IV transaminases Haliangium ochraceum
2.6.1.42 evolution the enzyme belongs to the PLP fold type IV transaminases Methanococcus vannielii
2.6.1.42 evolution the enzyme belongs to the PLP fold type IV transaminases Thermomicrobium roseum
2.6.1.42 evolution the enzyme belongs to the PLP fold type IV transaminases Thermobaculum terrenum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.B21 0.016
-
 (R)-1-phenylethylamine pH 9.0, 40°C, recombinant enzyme, with 3-methyl-2-oxovalerate Haliangium ochraceum
2.6.1.42 0.019
-
 (R)-1-phenylethylamine pH 9.0, 50°C, recombinant enzyme, with 2-oxogluarate Thermobaculum terrenum