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Literature summary extracted from

  • Aslam, M.; Takahashi, N.; Matsubara, K.; Imanaka, T.; Kanai, T.; Atomi, H.
    Identification of the glucosamine kinase in the chitinolytic pathway of Thermococcus kodakarensis (2018), J. Biosci. Bioeng., 125, 320-326 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.147 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.147 D-glucosamine
-
 Thermococcus kodakarensis
2.7.1.147 D-glucose
-
 Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 0.08
-
 ADP pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 0.08
-
 ADP at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 0.33
-
 D-glucosamine pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 0.33
-
 D-glucosamine at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 0.77
-
 D-glucose pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 0.77
-
 D-glucose at pH 7.5 and 80°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.147 ADP + D-glucosamine Thermococcus kodakarensis the enzyme functions as the glucosamine kinase responsible for the chitin degradation AMP + D-glucosamine 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucosamine Thermococcus kodakarensis ATCC BAA-918 the enzyme functions as the glucosamine kinase responsible for the chitin degradation AMP + D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Thermococcus kodakarensis
-
-
-
2.7.1.147 Thermococcus kodakarensis Q5JE39
-
-
2.7.1.147 Thermococcus kodakarensis ATCC BAA-918 Q5JE39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147
-
 Thermococcus kodakarensis
2.7.1.147 Resource Q column chromatography and Superdex S200 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucosamine
-
 Thermococcus kodakarensis AMP + D-glucosamine 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucosamine the enzyme functions as the glucosamine kinase responsible for the chitin degradation Thermococcus kodakarensis AMP + D-glucosamine 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucosamine
-
 Thermococcus kodakarensis ATCC BAA-918 AMP + D-glucosamine 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucosamine the enzyme functions as the glucosamine kinase responsible for the chitin degradation Thermococcus kodakarensis ATCC BAA-918 AMP + D-glucosamine 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus kodakarensis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus kodakarensis ATCC BAA-918 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + N-acetyl-D-glucosamine the enzyme exhibits a low level of activity against N-acetyl-D-glucosamine Thermococcus kodakarensis AMP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.147 additional information activity is not observed toward chitobiose and N-,N-diacetylchitobiose Thermococcus kodakarensis ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.147 ? x * 48000, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 GlcN kinase
-
 Thermococcus kodakarensis
2.7.1.147 glucosamine kinase
-
 Thermococcus kodakarensis
2.7.1.147 TK1110
-
 Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.147 120
-
 D-glucosamine pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 120
-
 D-glucosamine at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 210
-
 ADP pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 210
-
 ADP at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 248
-
 D-glucose pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 248
-
 D-glucose at pH 7.5 and 80°C Thermococcus kodakarensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.147 23
-
 D-glucose at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 23
-
 D-glucose pH 7.5, 70°C Thermococcus kodakarensis
2.7.1.147 60
-
 D-glucosamine at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 60
-
 D-glucosamine pH 7.5, 70°C Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
2.7.1.147 malfunction TK1110 disruption results in almost complete impairment in chitin degradation, and a complete loss of chitin-dependent H2 production Thermococcus kodakarensis
2.7.1.147 metabolism enzyme TK1110 functions as the glucosamine kinase responsible for the chitin degradation in Thermococcus kodakarensis Thermococcus kodakarensis
2.7.1.147 physiological function the enzyme functions as the glucosamine kinase responsible for the chitin degradation Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.147 320
-
 D-glucose at pH 7.5 and 80°C Thermococcus kodakarensis
2.7.1.147 360
-
 D-glucosamine at pH 7.5 and 80°C Thermococcus kodakarensis