Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Franceus, J.; Capra, N.; Desmet, T.; Thunnissen, A.
    Structural comparison of a promiscuous and a highly specific sucrose 6F-phosphate phosphorylase (2019), Int. J. Mol. Sci., 20, 3906 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.329 expression in Escherichia coli Ilumatobacter coccineus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.329 structure at 2.05 A resolution, comparison with structure of the Thermoanaerobacterium thermosaccharolyticum enzyme. The residues participating in hydrogen bonds with the phosphate are Arg152, Lys364, Lys434, and Tyr377 Ilumatobacter coccineus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.329 K364S mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
2.4.1.329 K434A strong decrease in kcat value Ilumatobacter coccineus
2.4.1.329 K434R mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
2.4.1.329 R152A mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus
2.4.1.329 Y377H mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value Ilumatobacter coccineus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.329 2
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 2.8
-
 D-fructose 6-phosphate mutant Y377H, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 3 3.2 D-fructose 6-phosphate mutant K434A, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 3.3
-
 D-fructose 6-phosphate mutant K152A, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 6.3
-
 D-fructose 6-phosphate mutant K364S, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 7.8
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
2.4.1.329 10.6
-
 D-fructose 6-phosphate mutant K434R, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 11.3
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
2.4.1.329 18.8
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.329 Ilumatobacter coccineus A0A6C7EEG6
-
-
2.4.1.329 Ilumatobacter coccineus YM16-304 A0A6C7EEG6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.329 45
-
 pH 6.5, 35°C Ilumatobacter coccineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.329 alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
-
 Ilumatobacter coccineus sucrose 6F-phosphate + phosphate
-
 r
2.4.1.329 alpha-D-glucose 1-phosphate + D-fructose 6-phosphate
-
 Ilumatobacter coccineus YM16-304 sucrose 6F-phosphate + phosphate
-
 r
2.4.1.329 additional information enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate Ilumatobacter coccineus ?
-
-
2.4.1.329 additional information enzyme displays strict specificity for substrates alpha-D-glucose 1-phosphate and beta-D-fructose 6-phosphate Ilumatobacter coccineus YM16-304 ?
-
-
2.4.1.329 sucrose 6F-phosphate + phosphate
-
 Ilumatobacter coccineus alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
-
 r
2.4.1.329 sucrose 6F-phosphate + phosphate
-
 Ilumatobacter coccineus YM16-304 alpha-D-glucopyranose 1-phosphate + D-fructofuranose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.329 ? x * 59900, SDS-PAGE, recombinant protein Ilumatobacter coccineus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.329 YM304_32550
-
 Ilumatobacter coccineus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.329 35
-
-
 Ilumatobacter coccineus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.329 0.61
-
 D-fructose 6-phosphate mutant K364S, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 0.8
-
 D-fructose 6-phosphate mutant Y377H, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 1.42
-
 D-fructose 6-phosphate mutant K152A, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 3.5
-
 D-fructose 6-phosphate mutant K434A, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 6.84
-
 D-fructose 6-phosphate mutant K434R, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 40
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 45
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 110
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
2.4.1.329 126
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.329 6
-
 phosphorolysis of sucrose 6F-phosphate Ilumatobacter coccineus
2.4.1.329 6.5
-
 synthesis of sucrose 6F-phosphate Ilumatobacter coccineus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.329 5.5 8 more than 50% of maximum activity, synthesis of sucrose 6F-phosphate Ilumatobacter coccineus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.329 2.4
-
 alpha-D-glucose 1-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus
2.4.1.329 11.2
-
 sucrose 6F-phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
2.4.1.329 14.1
-
 phosphate wild-type, pH 6.0, 35°C Ilumatobacter coccineus
2.4.1.329 20
-
 D-fructose 6-phosphate wild-type, pH 6.5, 35°C Ilumatobacter coccineus