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Literature summary extracted from

  • Hoppe, C.M.; Albuquerque-Wendt, A.; Bandini, G.; Leon, D.R.; Shcherbakova, A.; Buettner, F.F.R.; Izquierdo, L.; Costello, C.E.; Bakker, H.; Routier, F.H.
    Apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family (2018), Glycobiology, 28, 333-343 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B72 gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Caenorhabditis elegans
2.4.1.B72 gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Plasmodium falciparum
2.4.1.B72 gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.B72 Amphomycin
-
 Plasmodium falciparum
2.4.1.B72 Amphomycin
-
 Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.B72 microsome
-
 Caenorhabditis elegans
-
-
2.4.1.B72 microsome
-
 Plasmodium falciparum
-
-
2.4.1.B72 microsome
-
 Toxoplasma gondii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B72 Mg2+ required Caenorhabditis elegans
2.4.1.B72 Mg2+ required Plasmodium falciparum
2.4.1.B72 Mg2+ required Toxoplasma gondii
2.4.1.B72 Mn2+ required Caenorhabditis elegans
2.4.1.B72 Mn2+ required Plasmodium falciparum
2.4.1.B72 Mn2+ required Toxoplasma gondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B72 additional information Caenorhabditis elegans the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide ?
-
-
2.4.1.B72 additional information Plasmodium falciparum the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide ?
-
-
2.4.1.B72 additional information Toxoplasma gondii the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-
2.4.1.B72 additional information Toxoplasma gondii VEG the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-
2.4.1.B72 additional information Toxoplasma gondii ATCC 50861 the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B72 Caenorhabditis elegans P34413
-
-
2.4.1.B72 Plasmodium falciparum C0H4S1
-
-
2.4.1.B72 Toxoplasma gondii B9QPM1
-
-
2.4.1.B72 Toxoplasma gondii ATCC 50861 B9QPM1
-
-
2.4.1.B72 Toxoplasma gondii VEG B9QPM1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.B72 tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B72 Ac-WAEW-NH2 peptide + GDP-mannose
-
 Caenorhabditis elegans ?
-
 ?
2.4.1.B72 Ac-WAEW-NH2 peptide + GDP-mannose
-
 Plasmodium falciparum ?
-
 ?
2.4.1.B72 Ac-WAEW-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii ?
-
 ?
2.4.1.B72 Ac-WAEW-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii VEG ?
-
 ?
2.4.1.B72 Ac-WAEW-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii ATCC 50861 ?
-
 ?
2.4.1.B72 Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
 Caenorhabditis elegans ?
-
 ?
2.4.1.B72 Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
 Plasmodium falciparum ?
-
 ?
2.4.1.B72 Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii ?
-
 ?
2.4.1.B72 Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii VEG ?
-
 ?
2.4.1.B72 Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
 Toxoplasma gondii ATCC 50861 ?
-
 ?
2.4.1.B72 additional information the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide Caenorhabditis elegans ?
-
-
2.4.1.B72 additional information the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide Plasmodium falciparum ?
-
-
2.4.1.B72 additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii ?
-
-
2.4.1.B72 additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii VEG ?
-
-
2.4.1.B72 additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii ATCC 50861 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B72 DPY-19
-
 Caenorhabditis elegans
2.4.1.B72 DPY-19
-
 Plasmodium falciparum
2.4.1.B72 DPY-19
-
 Toxoplasma gondii
2.4.1.B72 Dpy-19-like C-mannosyltransferase UniProt Plasmodium falciparum
2.4.1.B72 dpy19
-
 Caenorhabditis elegans
2.4.1.B72 dpy19
-
 Plasmodium falciparum
2.4.1.B72 dpy19
-
 Toxoplasma gondii
2.4.1.B72 Dumpy-19 protein homologue UniProt Toxoplasma gondii
2.4.1.B72 protein dumpy-19 UniProt Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B72 37
-
 assay at Caenorhabditis elegans
2.4.1.B72 37
-
 assay at Plasmodium falciparum
2.4.1.B72 37
-
 assay at Toxoplasma gondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B72 7.5
-
 assay at Caenorhabditis elegans
2.4.1.B72 7.5
-
 assay at Plasmodium falciparum
2.4.1.B72 7.5
-
 assay at Toxoplasma gondii

General Information

EC Number General Information Comment Organism
2.4.1.B72 malfunction a DPY-19 mutant shows a dumpy phenotype and a defect in Q neuroblast migration Caenorhabditis elegans
2.4.1.B72 physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family Caenorhabditis elegans
2.4.1.B72 physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Plasmodium falciparum Dpy19 is a C-mannosyltransferase Plasmodium falciparum
2.4.1.B72 physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase Toxoplasma gondii