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Literature summary extracted from

  • Fan, W.; Guo, Q.; Liu, C.; Liu, X.; Zhang, M.; Long, D.; Xiang, Z.; Zhao, A.
    Two mulberry phytochelatin synthase genes confer zinc/cadmium tolerance and accumulation in transgenic Arabidopsis and tobacco (2018), Gene, 645, 95-104 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 gene PCS1, two Morus notabilis PCS genes are identified based on a genome-wide analysis of the Morus genome database, DNA and amino acid sequence determination and analysis, exon-intron structures of the MnPCS genes, quantitative real-time PCR enzyme expression analysis, expression in Arabidopsis thaliana Morus notabilis
2.3.2.15 gene PCS2, two Morus notabilis PCS genes are identified based on a genome-wide analysis of the Morus genome database, DNA and amino acid sequence determination and analysis, exon-intron structures of the MnPCS genes, quantitative real-time PCR enzyme expression analysis, recombinant expression in Arabidopsis thaliana Morus notabilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 additional information Zn2+/Cd2+ concentrations in both shoots and roots of the transgenic Arabidopsis seedlings expressing PCS1 and/or PCS2 are higher than in wild-type seedlings at two different Zn2+/Cd2+ concentrations. In addition, there is a positive correlation between Zn2+ accumulation and the expression level of MnPCS1 or MnPCS2 Morus notabilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.15 cytosol
-
 Morus notabilis 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Morus notabilis
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Morus notabilis W9QSJ2
-
-
2.3.2.15 Morus notabilis W9QSW3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.15 root
-
 Morus notabilis
-
2.3.2.15 seedling
-
 Morus notabilis
-
2.3.2.15 shoot
-
 Morus notabilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Morus notabilis Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 MnPCS1
-
 Morus notabilis
2.3.2.15 MnPCS2
-
 Morus notabilis
2.3.2.15 PCS
-
 Morus notabilis
2.3.2.15 PCS1
-
 Morus notabilis
2.3.2.15 PCS2
-
 Morus notabilis
2.3.2.15 phytochelatin synthase
-
 Morus notabilis

Expression

EC Number Organism Comment Expression
2.3.2.15 Morus notabilis expression of PCS1 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+ up
2.3.2.15 Morus notabilis expression of PCS2 is induced in root, stem, and leaf tissues within 24 h of exposure to 0.3 mM Zn2+ and 0.03-0.1 mM Cd2+ up

General Information

EC Number General Information Comment Organism
2.3.2.15 physiological function phytochelatin synthase (PCS) is an enzyme involved in the synthesis of phytochelatins, cysteine-rich peptides which play a key role in heavy metal (HM) detoxification of plants. Mulberry has the potential to remediate HM-contaminated soils. The two PCS genes in Morus notabilis (PCS1 and 2) are involved in HM detoxification in Morus. MnPCS1 plays a more important role in Cd detoxification than MnPCS2. MnPCS enzymes can markedly increase the tolerance of the transgenic plants at high concentrations of Zn2+, while the corresponding relationship between Cd2+ accumulation and the expression of MnPCSs are not observed. There might be some factor-dependent posttranscriptional regulation of PCS, like intron-mediated enhancement, and/or an optimum PCS level for tolerance to and accumulation of HMs in plants Morus notabilis
2.3.2.15 physiological function phytochelatin synthase (PCS) is an enzyme involved in the synthesis of phytochelatins, cysteine-rich peptides which play a key role in heavy metal (HM) detoxification of plants. Mulberry has the potential to remediate HM-contaminated soils. The two PCS genes in Morus notabilis (PCS1 and 2) are involved in HM detoxification in Morus. MnPCS1 plays a more important role in Cd detoxification than MnPCS2. The enzymes can markedly increase the tolerance of the transgenic plants at high concentrations of Zn2+, while the corresponding relationship between Cd2+ accumulation and the expression of MnPCSs are not observed. There might be some factor-dependent posttranscriptional regulation of PCS, like intron-mediated enhancement, and/or an optimum PCS level for tolerance to and accumulation of HMs in plants Morus notabilis