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Literature summary extracted from

  • Meng, X.; Gangoiti, J.; de Kok, N.; van Leeuwen, S.S.; Pijning, T.; Dijkhuizen, L.
    Biochemical characterization of two GH70 family 4,6-alpha-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655 (2018), Food Chem., 253, 236-246 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B34 gene gtfX, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Ligilactobacillus aviarius
2.4.1.B34 gene gtfY, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Ligilactobacillus aviarius

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B34 additional information construction of deletion mutant GtfX-DELTANDELTAC encoding residues 448-1304, determination of the mutant product specificity, 1HNMR analysis is performed with the product mixtures obtained from MOS DP3-7, amylose V, starch, and amylopectin Ligilactobacillus aviarius
2.4.1.B34 additional information construction of deletion mutant GtfY-DELTANDELTAC encoding residues 438-1290, determination of the mutant product specificity, 1HNMR analysis is performed with the product mixtures obtained from MOS DP3-7, amylose V, starch, and amylopectin Ligilactobacillus aviarius

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B34 Ligilactobacillus aviarius
-
-
-
2.4.1.B34 Ligilactobacillus aviarius DSM 20655
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.B34 recombinant soluble His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Ligilactobacillus aviarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.B34 17.9
-
 purified recombinant enzyme mutant GtfX-DELTANDELTAC, pH 4.0, 53°C, substrate 0.125% w/v amylose V Ligilactobacillus aviarius
2.4.1.B34 19.6
-
 purified recombinant enzyme mutant GtfY-DELTANDELTAC, pH 5.0, 62°C, substrate 0.125% w/v amylose V Ligilactobacillus aviarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B34 amylopectin + acceptor transglycosylation activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 amylopectin + H2O hydrolytic activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 amylopectin + H2O hydrolytic activity Ligilactobacillus aviarius DSM 20655 ?
-
 ?
2.4.1.B34 amylose V + acceptor transglycosylation activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 amylose V + H2O hydrolytic activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 amylose V + H2O hydrolytic activity Ligilactobacillus aviarius DSM 20655 ?
-
 ?
2.4.1.B34 additional information mutant GtfX-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfX-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfX-DELTANDELTAC from amylose V, overview Ligilactobacillus aviarius ?
-
-
2.4.1.B34 additional information mutant GtfY-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfY-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfY-DELTANDELTAC from amylose V, overview Ligilactobacillus aviarius ?
-
-
2.4.1.B34 additional information mutant GtfX-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfX-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfX-DELTANDELTAC from amylose V, overview Ligilactobacillus aviarius DSM 20655 ?
-
-
2.4.1.B34 additional information mutant GtfY-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfY-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfY-DELTANDELTAC from amylose V, overview Ligilactobacillus aviarius DSM 20655 ?
-
-
2.4.1.B34 starch + acceptor transglycosylation activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 starch + H2O hydrolytic activity Ligilactobacillus aviarius ?
-
 ?
2.4.1.B34 starch + H2O hydrolytic activity Ligilactobacillus aviarius DSM 20655 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B34 ? x * 100000, about, recombinant enzyme mutant GtfX-DELTANDELTAC, SDS-PAGE Ligilactobacillus aviarius
2.4.1.B34 ? x * 100000, about, recombinant enzyme mutant GtfY-DELTANDELTAC, SDS-PAGE Ligilactobacillus aviarius

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B34 4,6-alpha-GT
-
 Ligilactobacillus aviarius
2.4.1.B34 GtfB-like 4,6-alpha-glucanotransferases
-
 Ligilactobacillus aviarius
2.4.1.B34 GtfX
-
 Ligilactobacillus aviarius
2.4.1.B34 GtfY
-
 Ligilactobacillus aviarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B34 53
-
 enzyme mutant GtfX-DELTANDELTAC Ligilactobacillus aviarius
2.4.1.B34 62
-
 enzyme mutant GtfY-DELTANDELTAC Ligilactobacillus aviarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.B34 30 55 enzyme mutant GtfX-DELTANDELTAC shows more than 60% of maximal activity within this temperature range Ligilactobacillus aviarius
2.4.1.B34 58 73 enzyme mutant GtfY-DELTANDELTAC shows more than 90% of maximal activity within this temperature range Ligilactobacillus aviarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B34 4
-
 enzyme mutant GtfX-DELTANDELTAC Ligilactobacillus aviarius
2.4.1.B34 5
-
 enzyme mutant GtfY-DELTANDELTAC Ligilactobacillus aviarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.B34 2.5 5.5 enzyme mutant GtfY-DELTANDELTAC shows more than 60% of maximal activity within this pH range Ligilactobacillus aviarius
2.4.1.B34 3 7 enzyme mutant GtfX-DELTANDELTAC shows more than 60% of maximal activity within this pH range Ligilactobacillus aviarius

General Information

EC Number General Information Comment Organism
2.4.1.B34 additional information structural modeling of Lactobacillus aviarius subsp. aviarius GtfX, compared to the crystal structure of Lactobacillus reuteri GtfB Ligilactobacillus aviarius
2.4.1.B34 additional information structural modeling of Lactobacillus aviarius subsp. aviarius GtfY, compared to the crystal structure of Lactobacillus reuteri GtfB Ligilactobacillus aviarius