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Literature summary extracted from

  • Chen, P.; Visokay, S.; Abrams, J.M.
    Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions (2020), Fly, 14, 3-9 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.16 gene gfat1, sequence comparison with isozyme GFAT2 Drosophila melanogaster
2.6.1.16 gene gfat2, sequence comparisons wit isozyme GFAT1 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.16 additional information Crispr-mediated elimination of Drosophila gfat genes and generation of single gene null mutations in the fly counterparts of gfat1 and gfat2 (gfat1-/- and gfat2-/-). Deletions for either enzyme are fully lethal and homozygotes lacking either GFAT1 or GFAT2 die at or prior to the first instar larval stage. When genetically eliminated, neither isoform is able to compensate for the other. Dietary supplementation with D-glucosamine-6-phosphate rescues GFAT2 deficiency and restores viability to gfat2-/- mutants. In contrast, glucosamine-6-phosphate does not rescue gfat1-/- animals Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate Drosophila melanogaster
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Drosophila melanogaster Q32KF3
-
-
2.6.1.16 Drosophila melanogaster Q9Y093
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.16 embryo
-
 Drosophila melanogaster
-
2.6.1.16 larva
-
 Drosophila melanogaster
-
2.6.1.16 pupa
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Drosophila melanogaster L-glutamate + D-glucosamine 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.16 GFAT
-
 Drosophila melanogaster
2.6.1.16 GFAT1
-
 Drosophila melanogaster
2.6.1.16 GFAT2
-
 Drosophila melanogaster
2.6.1.16 glutamine: fructose-6-phosphate amidotransferase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.16 25
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 7.5
-
 assay at Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.6.1.16 malfunction deletion of the isozyme is lethal Drosophila melanogaster
2.6.1.16 metabolism glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P) Drosophila melanogaster
2.6.1.16 additional information the residues involved in substrate binding are conserved in both Drosophila melanogaster GFAT1 and GFAT2 isozymes Drosophila melanogaster