EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.6 | synthesis | TbPDT is proficient in the biosynthesis of numerous therapeutic nucleosides, including didanosine, vidarabine, cladribine, fludarabine, and nelarabine. TbPDT has good potential as an industrial biocatalyst for the synthesis of a wide range of therapeutic nucleosides through an efficient and environmentally friendly methodology | Trypanosoma brucei brucei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.6 | gene pdt, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.6 | additional information | the structure-guided replacement of Val11 with either Ala or Ser results in variants with 2.8fold greater activity. TbPDT is covalently immobilized on glutaraldehyde-activated magnetic microspheres. MTbPDT3 is selected as the best derivative (4200 IU/g, activity recovery of 22%), and can be easily recaptured and recycled for over 25 reactions with negligible loss of activity. TbPDT is immobilized onto magnetic microspheres, which are physically characterized by scanning electron microscopy and dynamic light scattering | Trypanosoma brucei brucei |
2.4.2.6 | V11A | site-directed mutagenesis, the mutant shows increased activity with 2'-deoxyinosine and adenine compared to wild-type enzyme | Trypanosoma brucei brucei |
2.4.2.6 | V11S | site-directed mutagenesis, the mutant shows increased activity with 2'-deoxyinosine and adenine compared to wild-type enzyme | Trypanosoma brucei brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.6 | additional information | - |
additional information | Michaelis-Menten kinetics | Trypanosoma brucei brucei | |
2.4.2.6 | 0.2 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 0.7 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 1.2 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 1.8 | - |
adenine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 2 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 2.9 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 2.9 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 5.1 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 6.8 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, nucleobase release | Trypanosoma brucei brucei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 39070 | - |
recombinant homodimeric enzyme, analytical centrifugation | Trypanosoma brucei brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei 927/4 GUTat10.1 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.6 | Trypanosoma brucei brucei | Q57VC7 | - |
- |
2.4.2.6 | Trypanosoma brucei brucei 927/4 GUTat10.1 | Q57VC7 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 9.5 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyadenosine and hypoxanthine | Trypanosoma brucei brucei |
2.4.2.6 | 10.5 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyadenosine and guanine | Trypanosoma brucei brucei |
2.4.2.6 | 11 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyinosine and guanine | Trypanosoma brucei brucei |
2.4.2.6 | 21.4 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyguanosine and adenine | Trypanosoma brucei brucei |
2.4.2.6 | 38.5 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyinosine and adenine | Trypanosoma brucei brucei |
2.4.2.6 | 39 | - |
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyguanosine and hypoxanthine | Trypanosoma brucei brucei |
2.4.2.6 | 50.3 | - |
purified recombinant mutant V11A, pH 6.0, 50°C, substrates 2'-deoxyguanosine and adenine | Trypanosoma brucei brucei |
2.4.2.6 | 59.9 | - |
purified recombinant mutant V11S, pH 6.0, 50°C, substrates 2'-deoxyguanosine and adenine | Trypanosoma brucei brucei |
2.4.2.6 | 84.7 | - |
purified recombinant mutant V11A, pH 6.0, 50°C, substrates 2'-deoxyinosine and adenine | Trypanosoma brucei brucei |
2.4.2.6 | 90.7 | - |
purified recombinant mutant V11S, pH 6.0, 50°C, substrates 2'-deoxyinosine and adenine | Trypanosoma brucei brucei |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.6 | -20°C, purified recombinant His-tagged enzyme, 180 days, about 23% activity remaining | Trypanosoma brucei brucei |
2.4.2.6 | -80°C, purified recombinant His-tagged enzyme, 180 days, about 23% activity remaining | Trypanosoma brucei brucei |
2.4.2.6 | 4°C, purified recombinant His-tagged enzyme, 180 days, 85% activity remaining | Trypanosoma brucei brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | 2',3'-dideoxyadenosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine-2',3'-dideoxyriboside + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2,6-diaminopurine-2',3'-dideoxyriboside + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 2-fluoro-2',3'-dideoxyadenosine + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-fluoro-2',3'-dideoxyadenosine + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 6-chloropurine | - |
Trypanosoma brucei brucei | 6-chloropurine-2',3'-dideoxyriboside + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 6-mercaptopurine | low activity | Trypanosoma brucei brucei | 6-mercaptopurine-2',3'-dideoxyriboside + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 6-methoxy-2',3'-dideoxyguanosine + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyadenosine + 6-methylpurine | low activity | Trypanosoma brucei brucei | 6-methylpurine-2',3'-dideoxyriboside + adenine | - |
? | |
2.4.2.6 | 2',3'-dideoxyinosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine-2',3'dideoxyriboside + inosine | - |
? | |
2.4.2.6 | 2',3'-dideoxyinosine + 6-chloropurine | low activity | Trypanosoma brucei brucei | 6-chloropurine-2',3'-dideoxyriboside + inosine | - |
? | |
2.4.2.6 | 2',3'-dideoxyinosine + 6-mercaptopurine | low activity | Trypanosoma brucei brucei | 6-mercaptopurine-2',3'-dideoxyriboside + inosine | - |
? | |
2.4.2.6 | 2',3'-dideoxyinosine + 6-methoxyguanine | low activity | Trypanosoma brucei brucei | 6-methoxy-2',3'-dideoxyguanosine + inosine | - |
? | |
2.4.2.6 | 2',3'-dideoxyinosine + 6-methylpurine | low activity | Trypanosoma brucei brucei | 6-methylpurine-2',3'-dideoxyriboside + inosine | - |
? | |
2.4.2.6 | 2'-deoxyadenosine + guanine | - |
Trypanosoma brucei brucei | 2'-deoxyguanosine + adenine | - |
? | |
2.4.2.6 | 2'-deoxyadenosine + hypoxanthine | - |
Trypanosoma brucei brucei | 2'-deoxyinosine + adenine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine-2'deoxyriboside + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 2-chloro-2'-deoxyadenosine + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 2-fluoro-2'-deoxyadenosine + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 6-chloropurine | - |
Trypanosoma brucei brucei | 6-chloropurine-2'-deoxyriboside + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 6-mercaptopurine | - |
Trypanosoma brucei brucei | 6-mercaptopurine-2'-deoxyriboside + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 6-methoxy-2'-deoxyguanosine + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + 6-methylpurine | - |
Trypanosoma brucei brucei | 6-methylpurine-2'-deoxyriboside + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + benzimidazole | - |
Trypanosoma brucei brucei | benzimidazole 2'-deoxyriboside + guanine | - |
? | |
2.4.2.6 | 2'-deoxyguanosine + hypoxanthine | - |
Trypanosoma brucei brucei | 2'-deoxyinosine + guanine | - |
? | |
2.4.2.6 | 2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2.4.2.6 | 2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2.4.2.6 | 2'-deoxyinosine + guanine | - |
Trypanosoma brucei brucei | 2'-deoxyguanosine + hypoxanthine | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine 2'-deoxyriboside + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 2-chloro-2'-deoxyadenosine + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 2-fluoro-2'-deoxyadenosine + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 6-chloropurine | low activity | Trypanosoma brucei brucei | 6-chloropurine 2'-deoxyriboside + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 6-mercaptopurine | - |
Trypanosoma brucei brucei | 6-mercaptopurine 2'-deoxyriboside + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 6-methoxyguanine 2'-deoxyriboside + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + 6-methylpurine | low activity | Trypanosoma brucei brucei | 6-methylpurine 2'-deoxyriboside + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + 2-fluoroinosine | - |
? | |
2.4.2.6 | 2-fluoro-2'-deoxyinosine + guanine | - |
Trypanosoma brucei brucei | 2'-deoxyguanosine + 2-fluoroinosine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2,6-diaminopurine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2-chloroadenine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2-fluoroadenine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 6-chloropurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-chloropurine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-methoxyguanine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + 6-methylpurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-methylpurine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + guanine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl guanine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl adenine + hypoxanthine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl hypoxanthine + adenine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2,6-diaminopurine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2-chloroadenine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 2-fluoroadenine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 6-chloropurine | low activity | Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-chloropurine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 6-mercaptopurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-mercaptopurine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-methoxyguanine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + 6-methylpurine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl 6-methylpurine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + adenine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl adenine + guanine | - |
? | |
2.4.2.6 | 9-beta-D-arabinofuranosyl guanine + hypoxanthine | - |
Trypanosoma brucei brucei | 9-beta-D-arabinofuranosyl hypoxanthine + guanine | - |
? | |
2.4.2.6 | additional information | no activity with theophylline, and with 2',3'-dideoxyadenosine/2-chloroadenine, or 2',3'-dideoxyinosine/2-fluoroadenine, or 9-beta-D-arabinofuranosyl adenine/6-mercaptopurine | Trypanosoma brucei brucei | ? | - |
- |
|
2.4.2.6 | additional information | no activity with theophylline, and with 2',3'-dideoxyadenosine/2-chloroadenine, or 2',3'-dideoxyinosine/2-fluoroadenine, or 9-beta-D-arabinofuranosyl adenine/6-mercaptopurine | Trypanosoma brucei brucei 927/4 GUTat10.1 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.6 | homodimer | 2 * 19000, recombinant enzyme, SDS-PAGE, 2 * 19090, sequence calculation | Trypanosoma brucei brucei |
2.4.2.6 | More | in general, a higher oligomerization state for NDTs is not essential for catalysis and/or stabilization of the active conformation. Binding energy analysis of the TbPDT dimer interface, overview | Trypanosoma brucei brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.6 | nucleoside 2'-deoxyribosyltransferase | - |
Trypanosoma brucei brucei |
2.4.2.6 | PDT | - |
Trypanosoma brucei brucei |
2.4.2.6 | purine nucleoside 2'-deoxyribosyltransferase | - |
Trypanosoma brucei brucei |
2.4.2.6 | Tb927.5.1360 | locus name | Trypanosoma brucei brucei |
2.4.2.6 | TbPDT | - |
Trypanosoma brucei brucei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 55 | - |
recombinant enzyme | Trypanosoma brucei brucei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 30 | 80 | activity range, recombinant enzyme, profile overview | Trypanosoma brucei brucei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.6 | 6.4 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 7.9 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 10.2 | - |
adenine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 14.3 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 17 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 23.4 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 23.8 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 39.6 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 40.7 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, nucleobase release | Trypanosoma brucei brucei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 6 | - |
recombinant enzyme | Trypanosoma brucei brucei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 4 | 10 | and below, activity range, recombinant enzyme, profile overview | Trypanosoma brucei brucei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.2.6 | additional information | molecular dynamics simulations. TbPDT is a homodimer which displays the typical 2'-deoxyribose binding site motif made up of two acidic residues from one subunit (Asp76 and Glu82) and one polar residue from the characteristic NLM motif in the neighboring monomer (Asn126#). The 5'-hydroxyl group of the incoming 2'-deoxynucleoside hydrogen bonds to the carboxylate of Asp76 and is hydrogen bonded by the carboxamide of Asn126#. The nucleophilic attack on C1' of the sugar is effected by the sidechain carboxylate of Glu82, which is held in position by hydrogen bonds donated by the phenol group of Tyr5 and the 3'-hydroxyl of the sugar. In the near-attack conformation one of the lone pairs of the M128# sulfur is poised to interact with the developing positive charge on the oxocarbenium ion of the transition state. The purine ring is sandwiched between the hydrophobic side chains of Val11 and Phe12, on one side, and those of Ile49 and Leu127# on the other side, whereas the nucleobase's N-3 atom is recognized by the carboxamide nitrogen of Asn53 | Trypanosoma brucei brucei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.6 | 5.67 | - |
adenine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 5.99 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 7.77 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 8.07 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 8.21 | - |
adenine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine | Trypanosoma brucei brucei | |
2.4.2.6 | 8.5 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 11.29 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release | Trypanosoma brucei brucei | |
2.4.2.6 | 11.92 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine | Trypanosoma brucei brucei | |
2.4.2.6 | 32 | - |
2'-deoxyinosine | pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine | Trypanosoma brucei brucei |