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Literature summary extracted from

  • Zhou, Y.; Li, W.; You, W.; Di, Z.; Wang, M.; Zhou, H.; Yuan, S.; Wong, N.K.; Xiao, Y.
    Discovery of Arabidopsis UGT73C1 as a steviol-catalyzing UDP-glycosyltransferase with chemical probes (2018), Chem. Commun. (Camb.), 54, 7179-7182 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.203 gene UGT73C1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.203 additional information
-
 additional information steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.203 Mg2+ required Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.203 Arabidopsis thaliana Q9ZQ99
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.203 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.203 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.203 additional information the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol Arabidopsis thaliana ?
-
-
2.4.1.203 UDP-alpha-D-glucose + 2-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]ethyl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
-
 Arabidopsis thaliana UDP + ?
-
 ?
2.4.1.203 UDP-alpha-D-glucose + prop-2-yn-1-yl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate determination and analysis of the substrate binding structure of UGT73C1, model, overview Arabidopsis thaliana UDP + ?
-
 ?
2.4.1.203 UDP-glucose + steviol determination and analysis of the substrate binding structure of UGT73C1, model, overview Arabidopsis thaliana UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.203 AtZOG1 UniProt Arabidopsis thaliana
2.4.1.203 cytokinin-O-glucosyltransferase 1 UniProt Arabidopsis thaliana
2.4.1.203 UDP-glycosyltransferase 73C1 UniProt Arabidopsis thaliana
2.4.1.203 UGT73C1
-
 Arabidopsis thaliana
2.4.1.203 zeatin O-glucosyltransferase 1 UniProt Arabidopsis thaliana
2.4.1.203 ZOG1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.203 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.203 7.8 8 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.4.1.203 additional information homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates Arabidopsis thaliana