Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, C.; Wu, Y.; Yu, H.; Shah, I.; Li, Y.; Zeng, J.; Liu, B.; Mills, D.; Chen, X.
    One-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus alpha1-2-fucosyltransferase (2016), Chem. Commun. (Camb.), 52, 3899-3902 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.344 synthesis enzyme is applied in an efficient one-pot multienzyme fucosylation system for high-yield synthesis of human blood group H antigens containing beta1-3-linked galactosides and an important human milk oligosaccharide lacto-N-fucopentaose I in preparative and gram scales Thermosynechococcus vestitus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.344
-
 Thermosynechococcus vestitus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.344 DTT 10 mM, slight inhibition Thermosynechococcus vestitus
2.4.1.344 EDTA 10 mM, slight inhibition Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.344 0.56
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.73
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.79
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.344 Mg2+ 5-20 mM, about 2fold increase in activity Thermosynechococcus vestitus
2.4.1.344 Mn2+ 5-20 mM, about 2fold increase in activity Thermosynechococcus vestitus
2.4.1.344 additional information no divalnet cation required Thermosynechococcus vestitus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.344 Thermosynechococcus vestitus Q8DK72
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.344 GDP-beta-L-fucose + beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone
-
 Thermosynechococcus vestitus GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone
-
 ?
2.4.1.344 additional information enzyme works well with type I (Galbeta1-3GlcNAcbetaOR) and its derivative (Galbeta1-3GlcNAcalphaOR), Type III (Galbeta1-3GalNAcalphaOR), and type IV (Galbeta1-3GalNAcbetaOR) acceptors for adding a fucose alpha1-2-linked to the terminal galactose residue. Type II (Galbeta1-4GlcNAcbetaOR) and type V (Galbeta1-4GlcbetaOR) glycans are not good acceptors Thermosynechococcus vestitus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.344 tll0994
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.344 30 45
-
 Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.344 0.005
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.333
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.483
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.344 additional information
-
 fucosyl donor hydrolysis activity is minimum in the pH range 5.0-8.0. An optimal pH at 9.5 is observed for the GDP-fucose hydrolysis activity Thermosynechococcus vestitus
2.4.1.344 4.5 6 broad Thermosynechococcus vestitus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.344 6.5
-
 about 85% of maximum activity Thermosynechococcus vestitus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.344 0.009
-
 GDP-beta-L-fucose donor hydrolysis reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.422
-
 beta-D-Gal-(1->3)-beta-D-GlcNAc-2-anthranilic acid aglycone pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus
2.4.1.344 0.602
-
 GDP-beta-L-fucose fucosyl transferase reaction, pH not specified in the publication, temperature not specified in the publication Thermosynechococcus vestitus