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Literature summary extracted from

  • Keenan, T.; Mills, R.; Pocock, E.; Budhadev, D.; Parmeggiani, F.; Flitsch, S.; Fascione, M.
    The characterisation of a galactokinase from Streptomyces coelicolor (2019), Carbohydr. Res., 472, 132-137 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 overexpression in Escherichia coli Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 Ca2+ 5 mM Streptomyces coelicolor
2.7.1.6 Cu2+ 5 mM Streptomyces coelicolor
2.7.1.6 EDTA 5 mM Streptomyces coelicolor
2.7.1.6 Mn2+ 5 mM Streptomyces coelicolor
2.7.1.6 Ni2+ 5 mM Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.035
-
 ATP pH 7.4, 37°C Streptomyces coelicolor
2.7.1.6 0.627
-
 alpha-D-galactose pH 7.4, 37°C Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Mg2+ 5 mM, required Streptomyces coelicolor
2.7.1.6 Zn2+ 5 mM, slight activation Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.6 ATP + alpha-D-galactose Streptomyces coelicolor involved in Leloir pathway ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose Streptomyces coelicolor A3(2) involved in Leloir pathway ADP + alpha-D-galactose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Streptomyces coelicolor Q9K3S8
-
-
2.7.1.6 Streptomyces coelicolor A3(2) Q9K3S8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + 3-deoxy-D-galactose 85% conversion after 2 h under optimised conditions Streptomyces coelicolor ADP + 3-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + 3-deoxy-D-galactose 85% conversion after 2 h under optimised conditions Streptomyces coelicolor A3(2) ADP + 3-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose involved in Leloir pathway Streptomyces coelicolor ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose 91% conversion after 2 h under optimised conditions Streptomyces coelicolor ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose involved in Leloir pathway Streptomyces coelicolor A3(2) ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose 91% conversion after 2 h under optimised conditions Streptomyces coelicolor A3(2) ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine 87% conversion after 2 h under optimised conditions Streptomyces coelicolor ADP + D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine 87% conversion after 2 h under optimised conditions Streptomyces coelicolor A3(2) ADP + D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + L-arabinose 10% conversion after 2 h under optimised conditions Streptomyces coelicolor ADP + L-arabinose 1-phosphate
-
 ?
2.7.1.6 ATP + N-acetyl-D-galactosamine 40% conversion after 2 h under optimised conditions Streptomyces coelicolor ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + N-acetyl-D-galactosamine 40% conversion after 2 h under optimised conditions Streptomyces coelicolor A3(2) ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 GALK
-
 Streptomyces coelicolor
2.7.1.6 SCO3136
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.6 45
-
-
 Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.6 5 50 5°C: about 60% of maximal activity, 55°C: about 50% of maximal activity Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 1.35
-
 ATP pH 7.4, 37°C Streptomyces coelicolor
2.7.1.6 1.35
-
 alpha-D-galactose pH 7.4, 37°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 4 10
-
 Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.6 4 10 pH 4.0: about 90% of maximal activity, pH 10.0: about 90% of maximal activity Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 2.15
-
 alpha-D-galactose pH 7.4, 37°C Streptomyces coelicolor
2.7.1.6 38.9
-
 ATP pH 7.4, 37°C Streptomyces coelicolor