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Literature summary extracted from

  • Liu, S.; Wang, M.; Du, G.; Chen, J.
    Improving the active expression of transglutaminase in Streptomyces lividans by promoter engineering and codon optimization (2016), BMC Biotechnol., 16, 75 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.2.13 food industry the enzyme is important in the food industry Streptomyces hygroscopicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 gene tg1, gene structure of tg1, the Streptomyces hygroscopicus TGase ORF is composed of a secretory signal peptide gene, a pro-peptide gene, and the mature TGase gene, the upstream and downstream sequences of the ORF contain a putative promoter and a putative terminator, respectively, recombinant enzyme expression from codon-optimized gene with engineered promoter in Streptomyces lividans strain TK24 yielding Streptomyces lividans TK24/pIJ86/tg1, the extracellular TGase is secreted. The complete promoter of TGase can be restricted to the sequence from -693 to -48 in tg1. Because the region from -198 to -148 negatively affects the expression, this region is deleted from the complete promoter (-693 to -48), which yields the TGase expression plasmid pTGO. Codon optimization of the TGase gene Streptomyces hygroscopicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 additional information deletion analysis of the TGase promoter, the pro-peptide is essential for the correct folding of Streptomyces TGase, TGase is usually expressed in an inactive pro-TGase form, which is then converted to active TGase by the addition of activating proteases in vitro Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 protein glutamine + alkylamine Streptomyces hygroscopicus
-
 protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine Streptomyces hygroscopicus WSH03-13
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces hygroscopicus P81453
-
-
2.3.2.13 Streptomyces hygroscopicus WSH03-13 P81453
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.13 proteolytic modification transglutaminases (TGase) are synthesized as a zymogen (pro-TGase) in Streptomyces sp., the pro-peptide is essential for the correct folding of Streptomyces TGase, TGase is expressed in an inactive pro-TGase form, which is then converted to active TGase by activating proteases Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 additional information transglutaminase (TGase) is an enzyme that exhibits several catalytic activities: the crosslinking of proteins by forming Nepsilon-(gamma-glutamyl) lysine bonds, the incorporation of polyamines into protein, and the deamidation of protein-bound glutamines Streptomyces hygroscopicus ?
-
-
2.3.2.13 additional information transglutaminase (TGase) is an enzyme that exhibits several catalytic activities: the crosslinking of proteins by forming Nepsilon-(gamma-glutamyl) lysine bonds, the incorporation of polyamines into protein, and the deamidation of protein-bound glutamines Streptomyces hygroscopicus WSH03-13 ?
-
-
2.3.2.13 protein glutamine + alkylamine
-
 Streptomyces hygroscopicus protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Streptomyces hygroscopicus WSH03-13 protein N5-alkylglutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 38000, recombinant enzyme, SDS-PAGE Streptomyces hygroscopicus

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TGase
-
 Streptomyces hygroscopicus
2.3.2.13 transglutaminase
-
 Streptomyces hygroscopicus