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Literature summary extracted from

  • Miyazawa, K.; Yoshimi, A.; Zhang, S.; Sano, M.; Nakayama, M.; Gomi, K.; Abe, K.
    Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall alpha-1,3-glucan synthase (2016), Biosci. Biotechnol. Biochem., 80, 1853-1863 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.183 additional information disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively Aspergillus oryzae
2.4.1.183 additional information disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively Aspergillus oryzae
2.4.1.183 additional information disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus oryzae
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus oryzae RIB 40
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus oryzae ATCC 42149
-
 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.183 Aspergillus oryzae Q2TXK6
-
-
2.4.1.183 Aspergillus oryzae Q2UEQ6
-
-
2.4.1.183 Aspergillus oryzae Q2UIS4
-
-
2.4.1.183 Aspergillus oryzae ATCC 42149 Q2TXK6
-
-
2.4.1.183 Aspergillus oryzae ATCC 42149 Q2UEQ6
-
-
2.4.1.183 Aspergillus oryzae ATCC 42149 Q2UIS4
-
-
2.4.1.183 Aspergillus oryzae RIB 40 Q2TXK6
-
-
2.4.1.183 Aspergillus oryzae RIB 40 Q2UEQ6
-
-
2.4.1.183 Aspergillus oryzae RIB 40 Q2UIS4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.183 mycelium
-
 Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus oryzae UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus oryzae RIB 40 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?
2.4.1.183 UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
 Aspergillus oryzae ATCC 42149 UDP + [alpha-D-glucosyl-(1->3)]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.183 agsA
-
 Aspergillus oryzae
2.4.1.183 agsB
-
 Aspergillus oryzae
2.4.1.183 agsC
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
2.4.1.183 malfunction disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain Aspergillus oryzae
2.4.1.183 malfunction disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain Aspergillus oryzae
2.4.1.183 additional information analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively Aspergillus oryzae
2.4.1.183 physiological function the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not Aspergillus oryzae