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Literature summary extracted from

  • Gama, S.R.; Balachandran, N.; Berti, P.J.
    Campylobacter jejuni KDO8P Synthase, its inhibition by KDO8P oxime, and control of the residence time of slow-binding inhibition (2018), Biochemistry, 57, 5327-5338 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 expression in Escherichia coli Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 3-deoxy-D-manno-octulosonate 8-phosphate oxime uncompetitive-like inhibition with respect to Mn2+ and competitive with respect to substrates, both fast-binding inhibition (Ki value 10 microM) and slow-binding inhibition (Ki* value 0.57 microM) Campylobacter jejuni
2.5.1.55 EDTA reversible inactivation Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.021
-
 D-arabinose 5-phosphate 37°C, pH 7.5 Campylobacter jejuni
2.5.1.55 0.65
-
 phosphoenolpyruvate 37°C, pH 7.5 Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Co2+ full reactivation of EDTA-treated enzyme Campylobacter jejuni
2.5.1.55 Mn2+ full reactivation of EDTA-treated enzyme, Km value 0.130 mM Campylobacter jejuni
2.5.1.55 additional information reactivation of EDTA-treated enzyme, in decreasing order: Mn2+ = Co2+ = Ni2+ = Fe2+ > Ca2+ = Mg2+ = Cd2+ > Zn2+ > Cu2+ Campylobacter jejuni
2.5.1.55 Ni2+ full reactivation of EDTA-treated enzyme Campylobacter jejuni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 123000
-
 gel filtration Campylobacter jejuni

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Campylobacter jejuni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 additional information the kinetic mechanism is sequential ordered ter ter, with Mn2+ binding first Campylobacter jejuni ?
-
-
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Campylobacter jejuni 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 tetramer 4 * 29300, calulated from sequence Campylobacter jejuni

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 Kdo8P synthase
-
 Campylobacter jejuni