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Literature summary extracted from

  • Balachandran, N.; Heimhalt, M.; Liuni, P.; To, F.; Wilson, D.J.; Junop, M.S.; Berti, P.J.
    Potent inhibition of 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase by DAHP oxime, a phosphate group mimic (2016), Biochemistry, 55, 6617-6629 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.54 in complex with inhibitor 3-deoxy-D-arabinoheptulosonate-7-phosphate oxime Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 3-deoxy-D-arabinoheptulosonate-7-phosphate oxime binding is competitive with respect to all three substrates.The oxime functional group, combined with two crystallographic waters, binds at the same location in the catalytic center as the phosphate group of the tetrahedral intermediate. DAHP oxime binds to only one subunit of each tight dimer Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.015
-
 D-erythrose 4-phosphate pH 7.5, 25°C Escherichia coli
2.5.1.54 0.14
-
 phosphoenolpyruvate pH 7.5, 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Mn2+ required, Km value 0.0055 mM for rapid equilibrium assumption Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli C3TIE2 isoform DAHPS(Phe)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 additional information kinetic mechanism is rapid equilibrium sequential ordered ter ter, with the essential divalent metal ion, Mn2+, binding first, followed by phosphoenolpyruvate and D-erythrose 4-phosphate Escherichia coli ?
-
-
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 AroG
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 16.4
-
 phosphoenolpyruvate pH 7.5, 25°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 0.0015
-
 3-deoxy-D-arabinoheptulosonate-7-phosphate oxime pH 7., 25°C Escherichia coli