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Literature summary extracted from

  • Ruszkowski, M.
    Guarding the gateway to histidine biosynthesis in plants Medicago truncatula ATP-phosphoribosyltransferase in relaxed and tense states (2018), Biochem. J., 475, 2681-2697 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expressed in Escherichia coli BL21 gold cells Medicago truncatula

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 native and SeMet-labeled enzyme, hanging drop vapor diffusion method, using 2.53 M NaCl, 100 mM Bis-Tris propane pH 6.3, and 10% (v/v) glycerol Medicago truncatula

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP competitive inhibition Medicago truncatula
2.4.2.17 L-histidine complete inhibition at 0.1 mM Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.182
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula
2.4.2.17 0.47
-
 ATP at pH 8.0 and 24°C Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.17 chloroplast
-
 Medicago truncatula 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ 10 mM used in assay conditions Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Medicago truncatula
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Medicago truncatula
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Medicago truncatula G7JFL4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 HisTrap resin column chromatography and Superdex S200 gel filtration Medicago truncatula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.17 leaf
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Medicago truncatula ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Medicago truncatula 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.17 homohexamer x-ray crystallography Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATP-PRT1
-
 Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 0.57
-
 ATP at pH 8.0 and 24°C Medicago truncatula
2.4.2.17 0.57
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.17 0.0061
-
 in the presence of 2 mM AMP, at pH 8.0 and 24°C Medicago truncatula L-histidine
2.4.2.17 0.0301
-
 in the absence of AMP, at pH 8.0 and 24°C Medicago truncatula L-histidine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.17 1.2
-
 ATP at pH 8.0 and 24°C Medicago truncatula
2.4.2.17 3.1
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula