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Literature summary extracted from

  • Wang, D.; Tian, Y.; Wei, D.; Jing, Y.; Niu, H.; Xie, K.; Song, Y.
    DNA damage-induced foci of E2 ubiquitin-conjugating enzyme are detectable upon co-transfection with an interacting E3 ubiquitin ligase (2016), Biochem. Genet., 54, 147-157 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.23 gene rad6a, the S-Flag-biotin (SFB)-tagged full length E2 is expressed in HEK-293T, HeLa, or U2OS cells, recombinant coexpression of Rad6b with E3 ubiquitin ligases RNF168 and RAD18 in E2-deficient MEF cells leading to formation of ionizing radiation-induced foci (IRIF) Homo sapiens
2.3.2.23 gene rad6b, the S-Flag-biotin (SFB)-tagged full length E2 is expressed in HEK-293T, HeLa, or U2OS cells, recombinant coexpression of Rad6b with E3 ubiquitin ligases RNF168 and RAD18 in E2-deficient MEF cells leading to formation of ionizing radiation-induced foci (IRIF) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.23 additional information DNA damage-induced foci of E2 ubiquitin-conjugating enzyme are detectable upon co-transfection with an interacting E3 ubiquitin ligase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.23 Homo sapiens P49459
-
-
2.3.2.23 Homo sapiens P63146
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.23 E2 ubiquitin-conjugating enzyme
-
 Homo sapiens
2.3.2.23 Rad6a
-
 Homo sapiens
2.3.2.23 RAD6B
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.2.23 physiological function ubiquitination process consists of three main steps: the first step is activation: ubiquitin is activated by an E1 ubiquitin-activating enzyme, which is dependent on ATP. The second step is conjugation: E2 ubiquitin-conjugating enzymes catalyze the transfer of ubiquitin from E1 to the active site cysteine of the E2 via a trans(thio)esterification reaction. The third step is ligation: E3 ubiquitin ligases catalyze the final step of the ubiquitination cascade. Most commonly, they create an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. E2 ubiquitin-conjugating enzymes RAD6A and RAD6B are interacting with E3 ubiquitin ligase RNF168 and RAD18 in response to DNA damage, recombinant coexpression and interaction analysis, overview. Following the localization of E3 enzymes to DNA damage sites, the E2 enzymes are recruited to these sites, where they catalyze protein ubiquitination. DNA damage-induced foci of E2 ubiquitin-conjugating enzyme require E3 ubiquitin ligase for its formation in mammalian cells. RNF168 or RAD18 recruit RAD6A and RAD6B to DNA damage sites, the interaction is specific, no other E3 ligases intecat with Rad6A/Rad6B Homo sapiens
2.3.2.23 physiological function ubiquitination process consists of three main steps: the first step is activation: ubiquitin is activated by an E1 ubiquitin-activating enzyme, which is dependent on ATP. The second step is conjugation: E2 ubiquitin-conjugating enzymes catalyze the transfer of ubiquitin from E1 to the active site cysteine of the E2 via a trans(thio)esterification reaction. The third step is ligation: E3 ubiquitin ligases catalyze the final step of the ubiquitination cascade. Most commonly, they create an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. E2 ubiquitin-conjugating enzymes RAD6A and RAD6B are interacting with E3 ubiquitin ligase RNF168 and RAD18 in response to DNA damage, recombinant coexpression and interaction analysis, overview. Following the localization of E3 enzymes to DNA damage sites, the E2 enzymes are recruited to these sites, where they catalyze protein ubiquitination. DNA damage-induced foci of E2 ubiquitin-conjugating enzyme require E3 ubiquitin ligase for its formation in mammalian cells. RNF168 or RAD18 recruit RAD6A and RAD6B to DNA damage sites, the interaction is specific, no other E3 ligases interact with Rad6A/Rad6B Homo sapiens