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Literature summary extracted from

  • Tan, J.M.E.; Cook, E.C.L.; van den Berg, M.; Scheij, S.; Zelcer, N.; Loregger, A.
    Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis (2019), Atherosclerosis, 281, 137-142 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.23 C94S site-directed mutagenesis, a catalytically inactive mutant Homo sapiens
2.3.2.23 additional information enzyme knockout by UBE2J2 siRNA in Hep-G2 cells. HEK293T cells lacking the E2 ubiquitin-conjugating enzymes UBE2J1, UBE2J2, and UBE2G2 are generated using a limited CRISPR/Cas9-based library. Hap1 cells lacking UBE2J2 are generated by CRISPR/Cas9-mediated genome editing Homo sapiens
2.3.2.23 additional information HEK293T cells lacking the E2 ubiquitin-conjugating enzymes UBE2J1, UBE2J2 and UBE2G2 are generated using a limited CRISPR/Cas9-based library Homo sapiens
2.3.2.23 additional information HEK293T cells lacking the E2 ubiquitin-conjugating enzymes UBE2J1, UBE2J2, and UBE2G2 are generated using a limited CRISPR/Cas9-based library Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine Homo sapiens
-
 [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.23 Homo sapiens P60604
-
-
2.3.2.23 Homo sapiens Q8N2K1
-
-
2.3.2.23 Homo sapiens Q9Y385
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.23 HAP-1 cell
-
 Homo sapiens
-
2.3.2.23 HEK-293T cell
-
 Homo sapiens
-
2.3.2.23 Hep-G2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.23 S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 Homo sapiens [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.23 UBE2G2
-
 Homo sapiens
2.3.2.23 Ube2J1
-
 Homo sapiens
2.3.2.23 UBE2J2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.2.23 malfunction in UBE2G2 knockout cells, sterol-stimulated degradation of squalene monooxygenase (SQLE) is partly attenuated, but that of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is abolished Homo sapiens
2.3.2.23 malfunction in UBE2J1 knockout cells, sterol-stimulated degradation of squalene monooxygenase (SQLE) and of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) are unaffected Homo sapiens
2.3.2.23 malfunction in UBE2J2 knockout cells, sterol-stimulated degradation of squalene monooxygenase (SQLE), but not that of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), is largely attenuated. RNAi-mediated silencing of UBE2J2 expression in HepG2 cells also attenuates sterol-stimulated degradation of SQLE in a proteasome-dependent manner Homo sapiens
2.3.2.23 physiological function MARCH6, an E3 ubiquitin ligase, specifically promotes cholesterol-stimulated ubiquitylation and subsequent proteasomal degradation of squalene monooxygenase (SQLE), but not of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR). The sterol-dependent degradation machinery makes use of distinct E2 ubiquitin conjugating enzymes Homo sapiens
2.3.2.23 physiological function UBE2J2 is a new regulator of cellular cholesterol homeostasis in mammalian cells. MARCH6, an E3 ubiquitin ligase, specifically promotes cholesterol-stimulated ubiquitylation and subsequent proteasomal degradation of squalene monooxygenase (SQLE), but not of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR). The sterol-dependent degradation machinery makes use of distinct E2 ubiquitin conjugating enzymes. The ability of UBE2J2 to support SQLE degradation critically depends on its enzymatic activity. UBE2J2 as an important partner of MARCH6 in cholesterol-stimulated degradation of SQLE, thereby contributing to the complex regulation of cellular cholesterol homeostasis Homo sapiens