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Literature summary extracted from

  • Crespo, N.; Sanchez-Murcia, P.A.; Gago, F.; Cejudo-Sanches, J.; Galmes, M.A.; Fernandez-Lucas, J.; Mancheno, J.M.
    2'-Deoxyribosyltransferase from Leishmania mexicana, an efficient biocatalyst for one-pot, one-step synthesis of nucleosides from poorly soluble purine bases (2017), Appl. Microbiol. Biotechnol., 101, 7187-7200 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.6 synthesis potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine Leishmania mexicana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6 gene pdt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana U1103
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana MHOM
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana 2001
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Leishmania mexicana GT
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Leishmania mexicana E9AWJ0
-
-
2.4.2.6 Leishmania mexicana 2001 E9AWJ0
-
-
2.4.2.6 Leishmania mexicana GT E9AWJ0
-
-
2.4.2.6 Leishmania mexicana MHOM E9AWJ0
-
-
2.4.2.6 Leishmania mexicana U1103 E9AWJ0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana 2',3'-dideoxyguanosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana U1103 2',3'-dideoxyguanosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana MHOM 2',3'-dideoxyguanosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana 2001 2',3'-dideoxyguanosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + guanine
-
 Leishmania mexicana GT 2',3'-dideoxyguanosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana 2',3'-dideoxyinosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana U1103 2',3'-dideoxyinosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana MHOM 2',3'-dideoxyinosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana 2001 2',3'-dideoxyinosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyadenosine + hypoxanthine high activity Leishmania mexicana GT 2',3'-dideoxyinosine + adenine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana U1103 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana MHOM 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana 2001 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + adenine
-
 Leishmania mexicana GT 2',3'-dideoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2',3'-dideoxyinosine + guanine low activity Leishmania mexicana 2',3'-dideoxyguanosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyadenosine + guanine
-
 Leishmania mexicana 2'-deoxyguanosine + adenine
-
 ?
2.4.2.6 2'-deoxyguanosine + adenine
-
 Leishmania mexicana 2'-deoxyadenosine + guanine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Leishmania mexicana 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Leishmania mexicana U1103 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Leishmania mexicana MHOM 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Leishmania mexicana 2001 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + adenine
-
 Leishmania mexicana GT 2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-deoxyinosine + guanine
-
 Leishmania mexicana 2'-deoxyguanosine + hypoxanthine
-
 ?
2.4.2.6 2'-fluoro-2'-deoxyadenosine + guanine
-
 Leishmania mexicana 2'-fluoro-2'-deoxyguanosine + adenine
-
 ?
2.4.2.6 2'-fluoro-2'-deoxyinosine + adenine high activity Leishmania mexicana 2'-fluoro-2'-deoxyadenosine + hypoxanthine
-
 ?
2.4.2.6 2'-fluoro-2'-deoxyinosine + guanine high activity Leishmania mexicana 2'-fluoro-2'-deoxyguanosine + hypoxanthine
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana U1103 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana MHOM 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana 2001 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Leishmania mexicana GT 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl adenine + guanine low activity Leishmania mexicana ?
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl adenine + hypoxanthine low activity Leishmania mexicana ?
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl guanine + adenine
-
 Leishmania mexicana ?
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl guanine + hypoxanthine low activity Leishmania mexicana ?
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl hypoxanthine + adenine
-
 Leishmania mexicana ?
-
 ?
2.4.2.6 9-beta-D-arabinofuranosyl hypoxanthine + guanine low activity Leishmania mexicana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 2'-deoxyribosyltransferase
-
 Leishmania mexicana
2.4.2.6 LmPDT
-
 Leishmania mexicana
2.4.2.6 PDT
-
 Leishmania mexicana
2.4.2.6 type I NDT
-
 Leishmania mexicana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 40 50
-
 Leishmania mexicana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.6 20 90 over 50% of maximal activity within this range Leishmania mexicana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.6 40
-
 purified recombinant enzyme LmPDT remains stable during 36 h at different pH values Leishmania mexicana
2.4.2.6 95
-
 purified recombinant enzyme LmPDT, 5 min, inactivation Leishmania mexicana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.6 7 8
-
 Leishmania mexicana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.6 4 10 over 6% of maximal activity within this range Leishmania mexicana

General Information

EC Number General Information Comment Organism
2.4.2.6 physiological function N-deoxyribosyltransferases catalyze the direct transfer of the 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor through a one-step reaction Leishmania mexicana