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Literature summary extracted from

  • He, Z.; Luo, L.; Keyhani, N.O.; Yu, X.; Ying, S.; Zhang, Y.
    The C-terminal MIR-containing region in the Pmt1 O-mannosyltransferase restrains sporulation and is dispensable for virulence in Beauveria bassiana (2017), Appl. Microbiol. Biotechnol., 101, 1143-1161 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.109 gene pmt1, recombinant expression of His-tagged full-length wild-type enzyme and of truncated mutant Pmt1DELTA311-902 in Pichia pastoris strain GS115 via transformation by Agrobacterium tumefaciens strain AGL-1, quantitative real-time RT-PCR expression and analysis Beauveria bassiana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information generation of a truncated mutant lacking the C-terminal Pmt1 MIR-containing region (Pmt1DELTA311-902) by targeted gene replacement and construction of the C-terminal Pmt1 MIR-containing region deletion strains and complementation strains. Construction and analysis of the C-terminal Pmt1 MIR-containing region-affected transcriptomes. Complementation by expression of wild-type enzyme. The mutant shows altered conidial yield, conidial germination, hydrophobicity and adhesion, phenotypes, overview Beauveria bassiana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 cell wall
-
 Beauveria bassiana 5618
-
2.4.1.109 membrane transmembrane helical structure Beauveria bassiana 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] Beauveria bassiana
-
 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] Beauveria bassiana ARSEF 2860
-
 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] Beauveria bassiana
-
 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] Beauveria bassiana ARSEF 2860
-
 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Beauveria bassiana J5JB66
-
-
2.4.1.109 Beauveria bassiana ARSEF 2860 J5JB66
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.109 samples containing both growing mycelia and aerial conidia are harvested from 1:4-diluted SDAY plates grown at 26°C for 15 days, recombinant His-tagged full-length wild-type enzyme and truncated mutant Pmt1DELTA311-902 purified from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, nickel affinity chromatography and gel filtration Beauveria bassiana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.109 conidium aerial Beauveria bassiana
-
2.4.1.109 mycelium
-
 Beauveria bassiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-seryl-[protein]
-
 Beauveria bassiana dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-seryl-[protein]
-
 Beauveria bassiana ARSEF 2860 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-seryl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein]
-
 Beauveria bassiana dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] substrates used are Dol-P-[3H]mannose and 3.5 mM acceptor peptide Ac-YATAV-NH2 Beauveria bassiana dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein]
-
 Beauveria bassiana ARSEF 2860 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?
2.4.1.109 dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] substrates used are Dol-P-[3H]mannose and 3.5 mM acceptor peptide Ac-YATAV-NH2 Beauveria bassiana ARSEF 2860 dolichyl phosphate + 3-O-(alpha-D-mannosyl)-L-threonyl-[protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 PMT1
-
 Beauveria bassiana
2.4.1.109 Pmt1 O-mannosyltransferase
-
 Beauveria bassiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.109 25
-
 assay at Beauveria bassiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.109 7.5
-
 assay at Beauveria bassiana

General Information

EC Number General Information Comment Organism
2.4.1.109 malfunction the lack of C-terminal Pmt1 MIR-containing region affects transcriptomes, altering the transcription of genes involved in asexual development, e.g. transcription patterns of seven conidiation-associated genes flbA-flbD, fluG, hyd1, and hyd2. Loss of the enzyme's C-terminal Pmt1 MIR-containing region affects mannosylation of the cell wall proteins. The Pmt1DELTA311-902 cells show significant increases in lectin binding when probed with either the ConA or the JAL lectins as compared with the wild-type strain. Increased binding of the WGA lectin is also detected or Pmt1delta311-902 cells, but no obvious difference in reactivity with GNL between the wild-type and Pmt1delta311-902 cells. The mutant shows altered conidial yield, conidial germination, hydrophobicity and adhesion. Phenotypes, overview. Hyphal bodies isolated from insects infected with Pmt1DELTA311-902 cells are elongated and significantly longer than their wild-type counterparts. In addition, hyphal branching is seen in numerous cells, a phenomenon rarely detected in wild-type (and complemented strain) samples Beauveria bassiana
2.4.1.109 additional information cell wall composition of wild-type strain and the Pmt1DELTA311-902 mutant strain, overview Beauveria bassiana
2.4.1.109 physiological function the C-terminal Pmt1 MIR-containing region affects transcriptomes. Requirement of the C-terminal Pmt1 MIR containing region on Pmt1 O-mannosyltransferase enzyme activity in vivo. The C-terminal MIR-containing domain of Pmt1 is involved in cell wall integrity, cell surface characteristics, and cell adhesion. But the Pmt1 C-terminus domain containing the MIR motifs is dispensable for fungal virulence Beauveria bassiana