Literature summary extracted from
Zhao, H.; Gao, H.; Ji, K.; Yan, B.; Li, Q.; Mo, S.; Zheng, M.; Ou, Q.; Wu, B.; Li, N.; Jiang, C.
Isolation and biochemical characterization of a metagenome-derived 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments (2019), AMB Express, 9, 19 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.54 |
expression in Escherichia coli |
uncultured bacterium |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.5.1.54 |
molecular modeling of structure. The monomeric structure of Aro1A is a (beta/alpha)8 barrel structure. Phosphoenolpyruvate combines with residues R60, Q116, S119, K141, and R171 through eight hydrogen bonds. D-erythrose 4-phosphate combines with residues R60, K65, Q116, S119, K141, and R171 through nine hydrogen bonds |
uncultured bacterium |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.54 |
0.0196 |
- |
phosphoenolpyruvate |
pH 8.0, 40°C |
uncultured bacterium |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.5.1.54 |
Ba2+ |
presence of Ba2+ stimulates the activity by more than 300% |
uncultured bacterium |
|
2.5.1.54 |
Ca2+ |
presence of Ca2+ stimulates the activity by more than 300% |
uncultured bacterium |
|
2.5.1.54 |
Mn2+ |
presence of Mn2+ stimulates the activity by more than 250%, Mn2+ is liganded by residues C36, H206, E232, and D243 |
uncultured bacterium |
|
2.5.1.54 |
additional information |
not stimulating: Zn2+, Mg2+ |
uncultured bacterium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.54 |
uncultured bacterium |
A0A482F152 |
metagenomic library from subtropical marine mangrove sediment |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.54 |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O |
- |
uncultured bacterium |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.54 |
? |
x * 37000, SDS-PAGE |
uncultured bacterium |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.54 |
Aro1A |
- |
uncultured bacterium |
2.5.1.54 |
Dax1 |
- |
uncultured bacterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.5.1.54 |
40 |
- |
- |
uncultured bacterium |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
2.5.1.54 |
30 |
47 |
more than 60% of maximum activity |
uncultured bacterium |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.54 |
17.3 |
- |
phosphoenolpyruvate |
pH 8.0, 40°C |
uncultured bacterium |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.5.1.54 |
8 |
- |
- |
uncultured bacterium |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.5.1.54 |
7.3 |
8.5 |
more than 60% of maximum activity |
uncultured bacterium |
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.54 |
880 |
- |
phosphoenolpyruvate |
pH 8.0, 40°C |
uncultured bacterium |
|