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Literature summary extracted from

  • Wons, E.; Mruk, I.; Kaczorowski, T.
    Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates (2018), Sci. Rep., 8, 8243 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 0.0000244
-
 double stranded DNA adenine with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000751
-
 double stranded DNA adenine with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000782
-
 double stranded DNA adenine with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000784
-
 double stranded DNA adenine with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000855
-
 double stranded DNA adenine with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.000089
-
 double stranded DNA adenine with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000912
-
 double stranded DNA adenine with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0000914
-
 double stranded DNA adenine with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0001023
-
 double stranded DNA adenine with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0001273
-
 double stranded DNA adenine with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.000143
-
 double stranded DNA adenine with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0002576
-
 single stranded DNA adenine with specific sequence AAGCTT, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00027
-
 S-adenosyl-L-methionine at pH 7.0 and 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Haemophilus influenzae the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Lactococcus cremoris the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Haemophilus influenzae RD the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Lactococcus cremoris W15 the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli E1585-68 the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-
2.1.1.72 Escherichia coli E1585-68
-
-
-
2.1.1.72 Haemophilus influenzae
-
-
-
2.1.1.72 Haemophilus influenzae RD
-
-
-
2.1.1.72 Lactococcus cremoris
-
-
-
2.1.1.72 Lactococcus cremoris W15
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT Escherichia coli ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') Escherichia coli ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Haemophilus influenzae ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Lactococcus cremoris ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Haemophilus influenzae RD ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine Lactococcus cremoris W15 ?
-
-
2.1.1.72 additional information the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT Escherichia coli E1585-68 ?
-
-
2.1.1.72 additional information the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') Escherichia coli E1585-68 ?
-
-
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Haemophilus influenzae S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Lactococcus cremoris S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Haemophilus influenzae RD S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Lactococcus cremoris W15 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site Escherichia coli E1585-68 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Haemophilus influenzae S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Lactococcus cremoris S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Haemophilus influenzae RD S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Lactococcus cremoris W15 S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + double stranded DNA adenine
-
 Escherichia coli E1585-68 S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + single stranded DNA adenine the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity Escherichia coli S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + single stranded DNA adenine the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity Escherichia coli E1585-68 S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 isospecific adenine DNA methyltransferase
-
 Haemophilus influenzae
2.1.1.72 isospecific adenine DNA methyltransferase
-
 Escherichia coli
2.1.1.72 isospecific adenine DNA methyltransferase
-
 Lactococcus cremoris
2.1.1.72 M.EcoVIII
-
 Escherichia coli
2.1.1.72 M.HindIII
-
 Haemophilus influenzae
2.1.1.72 M.LlaCI
-
 Lactococcus cremoris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.72 0.0002
-
 double stranded DNA adenine with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00022
-
 double stranded DNA adenine with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00022
-
 double stranded DNA adenine with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00025
-
 double stranded DNA adenine with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00027
-
 double stranded DNA adenine with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00033
-
 double stranded DNA adenine with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00033
-
 double stranded DNA adenine with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00033
-
 double stranded DNA adenine with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.00033
-
 double stranded DNA adenine with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0005
-
 single stranded DNA adenine with specific sequence AAGCTT, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0015
-
 double stranded DNA adenine with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 0.0021
-
 double stranded DNA adenine with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.72 1.7
-
 double stranded DNA adenine with specific sequence AAACTT/TTTGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 1.7
-
 double stranded DNA adenine with specific sequence AGGCTT/TCCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 1.9
-
 double stranded DNA adenine with specific sequence AACCTT/TTGGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 1.9
-
 single stranded DNA adenine with specific sequence AAGCTT, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 2.2
-
 double stranded DNA adenine with specific sequence TAGCTT/ATGCAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 2.6
-
 double stranded DNA adenine with specific sequence ACGCTT/TGCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 2.7
-
 double stranded DNA adenine with specific sequence ATGCTT/TACGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 2.9
-
 double stranded DNA adenine with specific sequence AATCTT/TTAGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 4.2
-
 double stranded DNA adenine with specific sequence CAGCTT/GTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 4.3
-
 double stranded DNA adenine with specific sequence GAGCTT/CTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 19.6
-
 double stranded DNA adenine with specific sequence AAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli
2.1.1.72 86.1
-
 double stranded DNA adenine with specific sequence MAGCTT/TTCGAA, at pH 7.0 and 37°C Escherichia coli